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Glycolysis

Biosci 106

QuestionAnswer
GLYCOLYSIS >basics -E harvesting -10steps -3irreversible -E investment/generation -glucose(6C)>>>2xpyruvate(3C) -in cytosol -noO2 -generates ATP
Catabolic -provide E for ATP -breakdown big molecules -food oxidised
Anabolic -requires E -small>bigmolecules
Dehydrogenase -Enzyme which removes H+ & oxidises substrate -NAD+/FAD reduced
Isomerases enzyme catalyses molecule to and isomer
Kinases transfers a P from ATP to a molecule
STEP 1: =R->P & Enzyme Glucose--->Glucose-6-Phosphate *hexo/glucokinase *1 ATP -traps G in cell -irreversible
STEP 2: R->P & Enzyme Glucose-6-Phosphate--->Fructose-6-Phosphate *Phosphoglucoisomerase -structural change
STEP 3: Fructose-6-Phosphate--->Fructosose-1,6-Biphosphate *phosphofructokinase -1 ATP
STEP 4: Fructose-1,6-phosphate---> Dihydroxyacetone phosphate (+G3P) *Fructobiphosphate Aldolase
STEP5: Didydroxyacetone phosphate---> Glyceraldehyde-3-Phosphate *Triose phosphate isomerase
STEP6: Glyceraldehyde-3-phosphate--->1,3-Phosphobiglycerate *Glyceraldehyde-3-phophate Dehydrogenase -NAD+>>>NADH
STEP7: 1,3-biphosphoglycerate--->3-phosphoglycerate *phosphoglycerate kinase -ATP formed
STEP8: 3-phosphosglycerate--->2-phosphoglycerate *Phosphoglycerate mutase -isomerisation
STEP9: 2-phosphoglycerate--->phosphoenolpyruvate *Enolase
STEP10: Phosphoeonol Pyruvate--->pyruvate *pyruvate kinase -ATP formed
irreversible steps 1, 3, 10
Generation phases -2ATP per 3C -2NADH+4ATP -step 7
Energy investment -2ATP -steps 1
Regulation -Phosphofructokinase -Pyruvate kinase
regulation: -step 3 -ATP -ve -ADP +ve
Regulation: -step 10 -F-1,6-BP +ve -ATP,AcetylCoA -ve
Created by: meglet
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