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Bio401 - Lec4
Micro Bio Lecture 4
| Question | Answer |
|---|---|
| Essential Nutrients | Are those that must be supplied from the environment (can be limiting). |
| Macronutrients | Needed in large quantities. Major elements in cell macromoluecles; C, O, H, N, P, S. Ions necessary for protein function; Mg, CA, Fe, K. |
| Micronutrients | Trace elements necessary for enzyme function; Co, Cu, Mn, Zn. |
| Growth Factors | Specific nutrients not required by all cells. |
| Minimal Medium | Contains only the compounds needed for an organism to grow. |
| Autotrophs | Assimilate Co2 as a carbon source and assemble into organic molecules (mainly sugars). |
| Heterotrophs | Use performed organic molecules-rely on other organisms to form them. |
| Phototrophs | Obtain energy from chemical reactions triggered by light. |
| Chemotrophs | Obtain energy from oxidation-reduction reactions. |
| Lithotrophs | Use inorganic molecules as a source of electrons. |
| Organotrophs | Use organic molecules. |
| Membrane Potential | Generated when chemical energy is used to pump protons outside of the cell. |
| Proton Motive force | When the H+ gradient plus the charge difference (voltage potential) form an electrochemical potential. |
| F1Fo ATP synthase | Uses potential energy stored to transport nutrients into the cell, drive flagellar rotation, and make ATP. |
| Permeases | Substrate specific carrier proteins |
| Facilitated Diffusion | Helps solutes move across a membrane from a region of high concentration to one of lower concentration. Does not use energy and cannot move a molecule against a gradient. |
| Coupled Transport Systems | Are those in which energy released by moving a driving ion down its gradient is used to move a solute up its gradient. |
| Symport | When the two molecules travel in the same direction. |
| Antiport | When the actively transported molecule moves in the direction opposite to the driving ion. |
| ABC Transporters | Largest family of energy driven transport systems and is an ATP binding cassette superfamily. Two types; Uptake ABC transporters (critical for transporting nutrients) and Efflux ABC transporters (used as multi-drug efflux pumps. |
| Siderophores | Specialized molecules secreted to bind ferric ion and transport it into the cell. |
| Group Translocation | Is the process that uses energy to chemically alter the substrate during its transport. |
| Phosphotransferase System (PTS) | Uses energy from phosphoenolpyruvate (PEP) to attach a phosphate to specific sugars. |
| Dilution Streaking | Dragging a loop across the surface of an agar plate. |
| Spread Plate | When tenfold serial dilutions are performed on a liquid culture. A small amount of each dilution is then plated. |
| Comeplex Media | Nutrient rich but poorly defined media. |
| Synthetic Media | Media that is precisely defined. |
| Enriched Media | Complex media to which specific blood components are added. |
| Selective Media | Favor the growth of one organism over another. |
| Differential Media | Exploit differences between two species that grow equally well. |
| Fluorescence-activated Cell Sorter (FACS) | Fluorescent cells are passed through a small orifice and then past a laser. Detecters measure light scatter in the forward direction (measure of particle size) and to the side (particle shape or granularity). |
| Pour Plate | Used to count viable cells. |
| Binary Fission | Most bacteria divide this way, where one parent cell splits into 2 equal daughter cells. Undergo exponential growth. |
| Generation TIme | The time it takes for a population to double. An constant interval by which bacteria divide based on an environment with unlimited resources. Interval can be dependent on species, medium, temp, pH. |
| Doubling Time | The generation time for cells in culture (the population of cells doubles over one generation). |
| Continuous Culture | Where all cells in a population achieve a steady state, which allows detailed study of bacterial physiology. |
| Chemostat | Ensures logarithmic growth by constantly adding and removing equal amounts of culture media. |
| Biofilms | Surface-attached communities of bacteria. Can be constructed by one or multiple species, and can form on a range of organic or inorganic surfaces. |
| Quarum Sensing | Communication with each other by sending and receiving chemical messengers. |
| Endospore | Produced by bacteria when faced with environmental stress. |
| Heterocysts | Specialized cells from cyanobacteria. Allow it to fix nitrogen anaerobically while maintaining oxygenic photosynthesis. |
| Gliding Motility | Energy-requiring process by which bacteria move smoothly over a solid surface. |
| Fruiting Body | A multicellular structure on which spore-producing structures are born. |