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Bio401 - Microscopy

Micro Bio Lecture 2 - Microscopy

QuestionAnswer
Dark-Field Microscopy -Enables microbes to be visualized as halos of bright light against darkness.
Phase-Contrast Microscopy -Exploits differences in the refractive index between the cytoplasm and surrounding medium or organelles.
Fluorescence Microscopy -Using fluorophore labeled antibodies to detect cells. -Certain fluorophores specifically bind to DNA
Confocal Microscopy -Images in high resolution, light is focused, and can visualize cells in three-dimensions if images are stacked.
Transmission Electron Microscopy -Electrons pass through the specimen and reveal internal structures.
Cyro-Electron Microscopy -Specimen is frozen and tomography is used to generate high-resolution models of virus particles.
Atomic Force Microscopy -Measures the van der Waals forces between electron shells of adjacent atoms of the cell surface and the sharp tip.
Scanning Electron Microscopy -Electrons scan the specimen surface and reveals external features in 3D
Bright-Field Microscopy -Generates a dark image of an object over a light background-block passage of light.
Created by: 11708312
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