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Bio401 - Lec2
Micro Bio Lecture 2
| Question | Answer |
|---|---|
| Dark-Field Microscopy | -Enables microbes to be visualized as halos of bright light against darkness. |
| Phase-Contrast Microscopy | -Exploits differences in the refractive index between the cytoplasm and surrounding medium or organelles. |
| Fluorescence Microscopy | -Using fluorophore labeled antibodies to detect cells. -Certain fluorophores specifically bind to DNA |
| Confocal Microscopy | -Images in high resolution, light is focused, and can visualize cells in three-dimensions if images are stacked. |
| Transmission Electron Microscopy | -Electrons pass through the specimen and reveal internal structures. |
| Cyro-Electron Microscopy | -Specimen is frozen and tomography is used to generate high-resolution models of virus particles. |
| Atomic Force Microscopy | -Measures the van der Waals forces between electron shells of adjacent atoms of the cell surface and the sharp tip. |
| Scanning Electron Microscopy | -Electrons scan the specimen surface and reveals external features in 3D |
| Bright-Field Microscopy | -Generates a dark image of an object over a light background-block passage of light. |
| Resolution | -The smallest distance by which two objects can be separated and still be distinguished. |
| Detection | -The ability to determine the presence of an object. |
| Magnification | -Means an increase in the apparent size of an image to resolve smaller separations between objects and increase the information obtained by the eye. |
| Electrong Microscopy | -Uses beams of electrons to resolve details. |
| Bacilli | -Rod shaped bacteria. |
| Cocci | -Sphere shaped bacteria. |
| Absorption | -Means that the photon's energy is acquired by the absorbing object. |
| Reflection | -Means that the wave front bounces off the surface of an object. |
| Refraction | -is the bending of light as it enters a substance that slows it speed. |
| Scattering | -Occurs when the wave front interacts with an object smaller than the wavelength of light. |
| Simple Stain | -Adds dark color specifically to cells but not to the external medium or surrounding tissue. |
| Differential Stain | -Stains one kind of cell, but not the other. |
| Gram Stain | -Differentiates between two types of bacteria. |
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