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Microbio Lab Test 1

QuestionAnswer
Compound Microscope Used to observe objects too small to see with the naked eye. Has 2 abilities: magnification(larger) and resolution(distinguish between objects close together).
Parfocal When you change the objective lenses the scope is still in focus.
Shapes of Bacteria Coccus=round/Bacillus=rod/spirilla=spiral.
Arrangements of bacteria Diplo=double/Strep=chain/Staphy=groups
Diaphragm Regulates light in microscope
Condenser Focuses light in microscope
Using Oil on microscope To preserve the direction of light rays at the highest magnification. It improves the resolving power of the lenses.
Aseptic Technique Is important to ensure the transfer of a pure culture.
Sterilization The process of rendering a medium or material free from all forms of life.
Subculturing Grow a pure line and test it.
Inoculating loop Used to xfer bacteria from broth, slants or plates.
Inoculating needle Used to xfer bacteria in agar deep.
3 forms of media Liquid, semisolid and solid.
Agar A solidifying agent. An extract of seaweed, which is a complex of carbs. Composed of galactose sugar.
Broth medium A liquid medium containing all the nutrients needed for bacteria growth.
Agar slant Tubes containing solid media at an angle.
Agar deep Contains a semi solid agar and an inoculating needle is used.
Pure Culture Contains a single type species of an organism.
Simple Staining Not too important. Used to creat contrast. Gives info about cell shape, size and arrangement. Positive dye used. Bacteria blue.
Negative Staining Stains the backround slide not the bacteria. Bacteria will appear clear. Same charge=repels. No heat fixing=less distortion of cell.
3 Stains for negative staining Negrosin, India Ink, Eosin blue.
Gram Staining The 1st test performed on bacteria. Devides bacteria into 2 groups. Gram positive & gram negative.
Differential Stain Distinguishes between different types of bacteria.
Crystal Violet Primary Dye. 1st step in gram staining.
Mordant Iodine. Increases the interaction between the bacterial cell wall & the crystal violet dye.
Alcohol Completely decolorize gram negative.
Safarin Counterstain. Will only stain the decolorized or gram negative cells.
Created by: nadiapr
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