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UCO

Urease, Catalase, Oxidase

QuestionAnswer
Urease Test How? Organism is grown in a urea broth medium containing the pH indicator phenol red
Urease Test Why? Substrate urea is split into its products and presence of ammonia creates alkaline environment causing phenol red to turn a deep pink.
Urease Test + and - results + = red color change, urea was degraded - = no color change, urea was not degraded
Urease test is the ability to degrade urea with enzyme urease
Catalase test How? Apply 3% hydrogen peroxide to organism and look for bubbles.
Catalase test Why? If hydrogen was hydrolyzed by the production of enzyme catalase because high amounts of h202 are deadly
Catalase Test + and - results + = bubbles, enzyme catalase is present - = no bubbles, no catalase enzyme is present
Catalase Test is to determine an organisms ability to degrade hydrogen peroxide by producing enzyme catalase.
Oxidase Test How? add test reagent p-aminodymethyline oxalate to colonies grown on a plate medium.
Oxidase Test Why? Reagant acts as an artificial substrate and donates electrons becoming oxidised.
Oxidase Test + and - results + = pink, marroon, to black, cytochrome oxidase production. - = no color change, lack of oxidase activity
Created by: wevarela
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