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micrbio identify

selective and differentative staining and culturing to identify unknowns

QuestionAnswer
S. pneumoniae Blood dark greenish colonies indicates alpha hemolysis.
S. pneumoniae Catalase there should be no bubbling since normal streptococci don’t have catalase.
S. pneumoniae MSA Streptococcus is inhibited by the high salt concentration.
S. aureus Blood clear halo around bacteria, indicative of Beta
S. aureus Catalase There should be bubbling since these bacteria have catalase
S. aureus MSA There should be growth since these bacteria are salt tolerant.
S. aureus Other Gelitinase positive.
S. pyogenes Blood there should be a clear halo around the growth, indicating beta
S. pyogenes Catalase there should be no bubbling, streptococcus is catalase negative.
S. pyogenes MSA there should be no growth as strep is salt intolerant.
S. epidermidis Blood there should be no halo or green around the growth as Staph epi is non(gamma) hemolytic.
S. epidermidis Catalase there should be bubbling since staphylococcus has catalase.
S. epidermidis MSA there should be growth as staph is salt tolerant, and also should be red media (no change) as Staph epi can’t ferment mannitol.
B. Subtilis Blood N/P (possibly beta).
B. Subtilis Catalase there should be bubbling because Bacillus subtilis has catalase.
B. Subtilis MSA N/P (would have been Salt tolerant).
B. Subtilis Other Caseinase+, Amylase+.
E. coli SS Should be inhibited, if present it would turn media pink because of the fermentation of lactose, should be no gas or black precipitate.
E. coli TSI slant and butt should be yellow because of glucose and lactose fermentation, may or may not be gas, no black precipitate (no H2S).
E. coli MAC Colonies/media red to acid production from lactose fermentation.
E. coli Citrate No growth and media remains green since no citrate utilization.
E. coli EMB Colonies should be dark blue/purple with a metallic sheen as a result of the high amounts of acid precipitating the dyes.
S. enterica SS growth with no production of red color to neutral red since Salmonella are paracolons, black precipitate.
S. enterica TSI yellow butt indicates acidic butt
S. enterica MAC media will remain colorless since lactose is not fermented, bile salt tolerant so will grow.
S. enterica Citrate Can utilize citrate as a sole source of carbon so media will be turned blue.
S. enterica EMB Colorless colonies as no lactose is fermented so no acid is generated to drive dyes out of solution.
K. pneumoniae SS inhibited, but if present pink colonies as a result of lactose fermentation, no H2S.
K. pneumoniae TSI butt = Acid/yellow, slant = Acid/yellow, gas varies, no H2S.
K. pneumoniae MAC bright pink colonies as a result of lactose fermentation.
K. pneumoniae Citrate blue media indicating citrate utilization as primary source of carbon.
K. pneumoniae EMB Metallic green/dark blue/purple as a result of heavy lactose fermentation.
Y. enterocolitica SS colorless colonies, no H2S
Y. enterocolitica TSI yellow/acid butt, red/basic slant(sometimes yellow from fermenting sucrose), gas production varies, no H2S.
Y. enterocolitica MAC Colorless colonies since lactose is not fermented.
Y. enterocolitica Citrate No color change as citrate cannot be utilized as a sole source of carbon.
Y. enterocolitica EMB Colorless colonies as no acid is produced from fermentation.
Organisms and results indicating positive for EMB metallic green/ dark blue, means acid from lactose fermentation precipitated dyes, += E. Coli, K. pneumoniae
Organisms and results indicating positive for SS if growth tolerant to brilliant green and bile salts. if black then S can be TEA, Salmonella forms black, E. Coli inhibited. Y. enterocolitica = growth but colorless
Organisms and results indicating positive for Mac Lactose fermentation makes media red, growth indicates tolerance to CV and bile salts, E. Coli, K. Pneumoniae = pink colonies, Salmonella & Y. entercolitica = colorless colonies
Organisms and results indicating positive for TSI Yellow butt = glucose fermentation at least, yellow slant = lactose &/or sucrose fermentation, Red slant only glucose, red butt obligate aerobe, black=S as TEA, bubble = H+ as TEA
Organisms and results indicating positive for Citrate Blue = Bromothymol blue acidified to blue bc of citrate utilization. K. Pneumonia & Salmonella
Organisms and results indicating positive for blood Beta=Staph Aureus & Strep pyogenes, Alpha = Strep pneumoniae, Gamma = Staph epi
Organisms and results indicating positive for catalase Staph and bacillus subtilis (not performed)
Organisms and results indicating positive for MSA staphs only grow, with aureus only turning yellow (mannitol fermentation)
Created by: alex01tib
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