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MolecularBiology12
| Question | Answer |
|---|---|
| target-primed reverse transcription | A process in which a free 3' end of a broken target DNA is used to prime a replication process, allowing a DNA copy to be made of an element that moves as a segment of RNA. |
| conservative site-specific recombination | A process in which a segment of DNA moves between specific recombination sites as DNA using a cognate recombinase. |
| transposition | A process in which a discrete DNA entity can move between DNA sites that lack homology using a self-encoded recombination protein called a transposase. |
| inverted repeats | Two copies of the same DNA sequence, which are in the opposite orientation on the same DNA molecule. |
| insertion sequences | A small bacterial transposon (several hundred nucleotide base pairs in length) that carries only the genes needed for its own transposition. |
| recombinase | An enzyme that acts at dif, converting a chromosome dimer into two separate daughter chromosomes. |
| autonomous elements | Transposons that encode both the transposase and the end sequences required to mobilize the element. |
| nonautonomous elements | Transposons that have the end sequence recognized by the transposase, but do not encode the transposase. |
| IS element | insertion sequence, the only gene it contains is tranposase (plus inverted repeats) |
| target site duplication | The process by which the direct repeats externally flanking the inverted repeats become duplicated upon insertion, with one copy at each end |
| integron system | A natural genetic system that encodes various antibiotic resistances and pathogenicity factors that can be found in plasmids, transposons, and the chromosome of bacteria. |
| transposon | sequences of DNA that can move or transpose themselves to new positions within the genome of a single cell |
| Transposase | enzyme that binds to the ends of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. |
| cut-and-paste transposition | The reaction pathway that results in the element excising from one DNA site and inserting into a new DNA site. |
| replicative transposition | copy of element is left at initial site. copy and paste |
| retrotransposition | Transposition via an RNA form in which DNA is transcribed into RNA, then reverse-transcribed into DNA, which is inserted at a new site in the genome. |