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MolecularBiology12

QuestionAnswer
target-primed reverse transcription A process in which a free 3' end of a broken target DNA is used to prime a replication process, allowing a DNA copy to be made of an element that moves as a segment of RNA.
conservative site-specific recombination A process in which a segment of DNA moves between specific recombination sites as DNA using a cognate recombinase.
transposition A process in which a discrete DNA entity can move between DNA sites that lack homology using a self-encoded recombination protein called a transposase.
inverted repeats Two copies of the same DNA sequence, which are in the opposite orientation on the same DNA molecule.
insertion sequences A small bacterial transposon (several hundred nucleotide base pairs in length) that carries only the genes needed for its own transposition.
recombinase An enzyme that acts at dif, converting a chromosome dimer into two separate daughter chromosomes.
autonomous elements Transposons that encode both the transposase and the end sequences required to mobilize the element.
nonautonomous elements Transposons that have the end sequence recognized by the transposase, but do not encode the transposase.
IS element insertion sequence, the only gene it contains is tranposase (plus inverted repeats)
target site duplication The process by which the direct repeats externally flanking the inverted repeats become duplicated upon insertion, with one copy at each end
integron system A natural genetic system that encodes various antibiotic resistances and pathogenicity factors that can be found in plasmids, transposons, and the chromosome of bacteria.
transposon sequences of DNA that can move or transpose themselves to new positions within the genome of a single cell
Transposase enzyme that binds to the ends of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism.
cut-and-paste transposition The reaction pathway that results in the element excising from one DNA site and inserting into a new DNA site.
replicative transposition copy of element is left at initial site. copy and paste
retrotransposition Transposition via an RNA form in which DNA is transcribed into RNA, then reverse-transcribed into DNA, which is inserted at a new site in the genome.
Created by: yayitzlauren
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