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Microbiology exam 1b

Metabolic Pathways

QuestionAnswer
Entner-Doudoroff pathway all archaea, some gram negative bacteria, has 6-phosphogluconate dehydrase not hexose isomerase, & 2-keto-3deoxyphosphogluconate aldolase not 1,6 PBG aldolase which creates pyruvate and Glyceraldehyde phosphate. Net 1 ATP, 1NADH, 1NADPH, 2 pyruvate
Pentose phosphate pathway generates NADPH, ribulose-5-phosphate for nucleotides, and erythrose-4-phosphate for aromatic amino acids
Phopshoketolase pathway alternative bacterial pathway for generating NADPH +H+
Homofermentive and heterofermentive lactate bacteria Homo-only lactate is made, hetero- form lactate,CO2,ethanol,acetate,formate Heterofermentive have fructose 1,6 bisphosphate aldolase
Propionibacterium converts lactate to proprionate (swiss cheese flavor), acetate, and CO2(swiss cheese holes)
Mixed acid fermentation produces lactate, ethanol, formate, succinate, acetate, CO2, and H2
Butanediol fermentation produces lactate, succinate, butanediol, ethanol , CO2, H2
Methyl red test tests for mixed acid fermentation pH--> Red < 5 < yellow
Voges-Proskauer test Detects acetoin, an intermediate to butanediol formation. (i.e. enterobacter)
Net products of Pyruvate to CO2 by aerobic respiration 3CO2, 4NADH+H+, 1FADH2, 1GTP,
ETC electron path NADH+H+->FADH2->CoQ->Cytb->cytc->cyta->O2
ETC/Ox-Phos inhibitors rotenone blocks complex 1, antimycin blocks cytb->cytc, azide/CN/CO block CytC->O2, oligomycin/atractyloside block phosphorylation
Siderophore molecule in niceria gonorrhoeae that binds iron better than hemoglobin
Temperature ranges for different types of bacteria -10-25C = Psychrophiles, 10-45C=Mesophiles, 30-80C=thermophiles, 80+C = extreme thermophiles
Created by: alex01tib
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