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bio ch. 11

Holt Biology Ch. 11

QuestionAnswer
Anode positive pole ( at the bottom)
Cathode negative pole (at the top)
DNA ligase an enzyme that joins the ends of the two types of cut DNA
EcoRi specific bacteria used to put into plasmids
Forensics Forensics is a field of science dedicated to the methodical gathering and analysis of evidence to establish facts that can be presented in a legal proceeding. Though crime scenes and laboratories
Gene cloning the process of isolating a gene sequence in the genome of an organism and inserting the gene sequence into a plasmid vector for production in large numbers
Humulin human insulin
Probes detection system for DNA
Restriction fragment length polymorphisms DNA fragments of different lengths; these are used to identify individuals and to determine how closely related members of a population are to one another
Restriction enzymes an enzyme that destroys foreign DNA molecules by cutting them at specific sites
Sticky ends the ends of the cut pieces of DNA (cut from the restriction enzymes) with short single strands on each end that are complementary to each other.
Bacterial transformation when u insert T recombinant plasmid into bacteria (T exchange of genetic material btwn strains of bacteria by T transfer of a fragment of naked DNA from a donor cell to a recipient cell, followed by recombination in T recipient chromosome.)
DNA fingerprint comparing DNA sample from crime scene with suspects and victim by comparing blood samples on defendant’s cloths.
Dr. Henry Lee he was recognized as one of the world’s leader in forensics
Esherichia coli(E.coli) a bacteria that is used as a bacteria to mass-produce many useful chemicals drugs ( treat diabetes, treatment for burns, treatment for ovarian cancer, ect)
Gel electrophoresis method of separating DNA in a gelatin like material using an electrical field.
Genetic engineering the process of manipulating genes for practical purposes
NCBI Gen Bank data base show sequence of human bases.
Plasmids small circular DNA molecules that replicate separately from the much larger bacteria chromosomes, which is small supplemental circles of DNA
Recombinant DNA DNA made from two or more different organisms.
Stanley Cohn and Herbert Boyer made 1st genetically altered organism. used an African clawed frog as their experimental organism, & they isolated an rRNA gene from one of its chromosomes, & then they inserted the gene into bacteria, & that bacteria produced frog rRNA.
Vector a way to carry human DNA into bacteria (carry one organism to another)
Argose type of gel protein that is used in electrophoresis
Created by: lezzy
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