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bio: DNA replication
key enzymes/proteins
| Question | Answer |
|---|---|
| helicase | unwinds parental double helix, unzips down center (starts at sections with A-T's because they have fewer bonds and are broken more easily |
| binding proteins | stabilize separated strands, prevents DNA from rezipping (not an enzyme) |
| primase | adds short RNA primer strand to template/parental strand |
| DNA polymerase | adds DNA nucleotides to build polymer |
| exonuclease | removes RNA primer and inserts correct DNA nucleotides |
| ligase | joins Okazaki fragments and seals nicks in sugar-phosphate backbone |
| quirks of DNA polymerase: | can only add nucleotides of 3-prime end of growing strand), can only add FIRST to RNA |