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techniques in cell
| Question | Answer |
|---|---|
| the complex process by which cells are grown under controlled conditions | Cell culture |
| biochemical technique in which a mixture of substances is separated by charge, size, or some property by allowing it to partition between a moving phase and a stationary phase | Chromatography |
| lysed cells crude extract | Homogenate |
| using current (negative charge) to separate macromolecules by size | Electrophoresis |
| is the reversible inclusion of a molecule (or group) between two other molecules (or groups | Intercalation |
| antigen binding molecule secreted by plasma cells | Antibody (aka immunoglobulin) |
| any molecule that can bind to immune system component (antibody, B cell receptor or T cell receptor) | Antigen |
| three dimensional region of an antigen that is recognized by the immune system (i.e. antibody) | Epitope |
| is a mixture of many different antibody molecules made against different epitopeson the same antigen | Polyclonal antibody |
| identical antibodies recognizing a single epitope | Monoclonal antibody |
| the technique of precipitating a protein and everything that protein is bound to out of solution using an antibody that specifically for that particular protein | Immunoprecipitation |
| a technique that can detect the presence of a specific proteins via antibodies | Western blot analysis |
| the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye | Microscopy |
| contrast in a bright-field light microscope is created by differences in the amount of light absorbed by different regions of the specimen | Contrast |
| how many times larger the object is made bigger | Magnification |
| the resolution of an optical system defines the closest proximity of two objects that can be seen as two distinct regions of the image | Resolution |
| separates certain organelles or cells based on density | Differential centrifugation |
| a laboratory device in which a sample is spun around a central axis at high speed. Used to separates suspended materials of different densities | Centrifuge |
| cell biology technique that allows the counting and analysis of cells in a liquid media | Flow cytometry |
| PS is a phospholipid that normally resides on the cytosolic side of the plasma membrane | •Phosphatidylserin |
Created by:
ksams7
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