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DNA Replication
| Question | Answer |
|---|---|
| is the transfer of genetic material between 2 bacteria through a bridge-like connection between the two cells called a sex pilus | Bacterial conjugation |
| special plasmids that can integrate into host chromosomes | Episome |
| mechanism for transfer of genetic information in bacteria through a cell surface receptor | transformation |
| In DNA, the total abundance of purines is equal to the total abundance of pyrimidine | Chargaff’s Rule |
| Purines | Adenine Guanine |
| Pyrimidine | • Cytosine • Thymine |
| Each parent strand serves as a template for a new strand | Semiconservative replication |
| Enzyme that uses energy from ATP hydrolysis to unwind the DNA | DNA helicase |
| Bind to unwound strands to keep them from re-associating into double helix | Single-strand binding proteins |
| Adds nucleotides to the growing chain – Two substrates • Nucleotides – • Template DNA | DNA polymerase |
| DNA polymerase cannot add new stands from scratch Starter strand – short single strand of RNA | Primer |
| Synthesizes the RNA primer stand | Primase |
| cut the DNA strand to alleviate the supercoiling and then ligate the DNA strands together | •Topoisomerase |
| in eukaryotes the repetitive sequence at the end of the chromosome | Telomere |
| DNA polymerase recognizing and removing improperly introduced nucleotides | DNA proofreading |
| Occurs after the DNA is replicated • Second set of proteins survey the newly replicated molecules for mispaired bases | Mismatch repair |
| the excision repair proteins excise the damaged base and some adjacent bases DNA polymerase1 adds the correct bases by 5 to 3 replication of the short strand | Excision Repair |