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Lab Microbiology
| Question | Answer |
|---|---|
| Group of bacteria forms a | Colony |
| Disease causing microorganisms | Pathogens |
| 4 types of pathogens | Fungi, Parasites, Viruses, Bacteria |
| Color of bacteria that stain gram positive | Purple |
| Mordant | Iodine |
| Retains safranin red | Gram Negative |
| Microorganisms normally found in the body and is not pathogenic | Normal Flora |
| In the absence of oxygen | Anaerobic |
| Study of fungi | Mycology |
| Requires living host to multiply and contains either RNA or DNA | Virus |
| Sexually transmitted disease sometimes found in urine | Trich |
| Test used to recover pinworms | Cellophane/Tape Test |
| Used to kill or inhibit the growth of a bacterial infection | Antibiotic |
| Total absence of microorganisms | Sterile |
| Contains ingredients that inhibit the growth of certain microorganisms | Selective Media |
| The media on which the specimen is first inoculated | Primary Media |
| Counterstain | Safranin |
| Requires very specific nutrient and environmental conditions | Fastidious |
| Decolorizer | Alcohol/Acetone |
| Stain used to differentiate bacteria by their cell walls | Gram Stain |
| Coccidioides, immitis, and Aspergillus are types of | Fungi |
| Beta hemolysis is characterized by | A clear zone around the colony. Complete destruction of erythrocytes |
| Beta hemolysis indicates | Group A Strep |
| Alpha hemolysis is characterized by | Green discoloration around the colony. Due to incomplete hemoglobin breakdown |
| Alpha hemolysis indicates | Group B Strep/Normal Flora |
| Test used to differentiate Staph from Strep | Oxidase Test (Hydrogen Peroxide) |
| Intestinal protozoan parasite that causes malabsorption syndrome | Giardia Lamblia |
| Parasitic worms are called | Helminth |
| Pathogenic bacteria found in a wound | Staphaureus |
| Purple gram stain reaction | Gram Positive |
| Patients should avoid aspirin and vitamin C for how long | 7 and 3 days |
| Test performed to differentiate staph organisms | Coagulase |
| Most common bacteria recovered from urine | E. coli |
| Specimen that is inoculated right from the source of infection | Direct Culture |
| Procedure to measure the growth of bacteria in the presence of antibiotics | Antibiotic Sensitivity/Susceptibility Testing |
| Scientific name for the pinworm | Enterobius Vermicularis |
| Test done for the most effective antibiotic | Kirby Bauer |
| Comma or Spiral shaped bacteria | Spirilla |
| Round or oval bacteria | Cocci |
| Rod-shaped bacteria | Bacilli |
| Cocci that appear singly | Micrococci |
| Cocci that appear in pairs | Diplococci |
| Cocci that appear in grapelike clusters | Staphylococci |
| Cocci that appear in chains | Streptococci |
| Pneumonia is caused by what type of bacteria (shape) | Cocci |
| Strep Throat is caused by what type of bacteria (shape) | Cocci |
| Wound Infection is caused by what type of bacteria (shape) | Cocci |
| Food Poisoning is caused by what type of bacteria (shape) | Cocci |
| Toxic Shock Syndrome is caused by what type of bacteria (shape) | Cocci |
| Gonorrhea is caused by what type of bacteria (shape) | Cocci |
| Meningitis is caused by what type of bacteria (shape) | Cocci |
| Bacillus found side by side like boards | Palisades |
| Bacillus attached end to end in chains | Streptobacilli |
| Botulism is caused by what type of bacteria (shape) | Bacilli |
| TB is caused by what type of bacteria (shape) | Bacilli |
| Typhoid fever is caused by what type of bacteria (shape) | Bacilli |
| Diptheria is caused by what type of bacteria (shape) | Bacilli |
| UTI is caused by what type of bacteria (shape) | Bacilli |
| Plague is caused by what type of bacteria (shape) | Bacilli |
| Legionnaires’ is caused by what type of bacteria (shape) | Bacilli |
| Tetanus is caused by what type of bacteria (shape) | Bacilli |
| Gangrene is caused by what type of bacteria (shape) | Bacilli |
| Syphilis is caused by what type of bacteria (shape) | Spirillum/Spirochete |
| Lyme Disease is caused by what type of bacteria (shape) | Spirillum/Spirochete |
| Pyloric Ulcers (H. Pylori) is caused by what type of bacteria (shape) | Spirillum/Spirochete |
| Food poisoning in US is caused by what type of bacteria (shape) | Spirillum/Spirochete |
| Streptococcus is gram | positive |
| Staphylococcus is gram | positive |
| Klebsiella is gram | negative |
| E. coli is gram | negative |
| Gonorrhea is gram | negative |
| Bacteria grow at what temp? | 37 Degrees Celsius |
| Incubate bacteria for how many hours? | 24-72 hours |
| Aerobic Bacteria | Require Oxygen |
| Anaerobic Bacteria | Don’t like Oxygen |
| Aspergillus is a type of | fungi |
| Candida is also known as | yeast |
| Coccidioides Immitis is also known as | valley fever |
| Skin disease caused by fungi | Tinea (Ring Worm), Athlete’s Foot, Jock Itch |
| Fungi causes what type of pneumonia | pneumocysitis |
| 4 Mycology Testing Methods | Skin scraping/swabs, KOH Prep, Wet Prep, Sabourad’s dextrose agar |
| Testing methods for virology | cell culture, ELISA, Kit tests (flue, herp, rubella) |
| Giardia lamblia is caused by | contaminated water |
| Plasmodium causes | malaria |
| Pubic lice is called | pthirus pubis |
| Head lice is called | pediculus humanus |
| Common intestinal parasites | Tape worm, round worm, hook worm, pin worm |
| Testing method for intestinal parasites | Formalin preserved stool specimen for microscopic exam |
| Testing method for malaria | thin & thick blood smear |
| Testing method for trich | Urine & Vaginal Exam |
| Contains substances that visibly change as a result of the metabolic activity of certain organisms | Indicator (Differential) Media |
| Types of selective and indicator media | EMB, MacConkey’s |
| CNA is a type of _________ media | selective |
| A culture that is grown from a single colony taken from a direct culture | Pure Culture |
| Swab of affected area is placed on a microscope slide and gram stained | Direct Smear |