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Lab Microbiology

QuestionAnswer
Group of bacteria forms a Colony
Disease causing microorganisms Pathogens
4 types of pathogens Fungi, Parasites, Viruses, Bacteria
Color of bacteria that stain gram positive Purple
Mordant Iodine
Retains safranin red Gram Negative
Microorganisms normally found in the body and is not pathogenic Normal Flora
In the absence of oxygen Anaerobic
Study of fungi Mycology
Requires living host to multiply and contains either RNA or DNA Virus
Sexually transmitted disease sometimes found in urine Trich
Test used to recover pinworms Cellophane/Tape Test
Used to kill or inhibit the growth of a bacterial infection Antibiotic
Total absence of microorganisms Sterile
Contains ingredients that inhibit the growth of certain microorganisms Selective Media
The media on which the specimen is first inoculated Primary Media
Counterstain Safranin
Requires very specific nutrient and environmental conditions Fastidious
Decolorizer Alcohol/Acetone
Stain used to differentiate bacteria by their cell walls Gram Stain
Coccidioides, immitis, and Aspergillus are types of Fungi
Beta hemolysis is characterized by A clear zone around the colony. Complete destruction of erythrocytes
Beta hemolysis indicates Group A Strep
Alpha hemolysis is characterized by Green discoloration around the colony. Due to incomplete hemoglobin breakdown
Alpha hemolysis indicates Group B Strep/Normal Flora
Test used to differentiate Staph from Strep Oxidase Test (Hydrogen Peroxide)
Intestinal protozoan parasite that causes malabsorption syndrome Giardia Lamblia
Parasitic worms are called Helminth
Pathogenic bacteria found in a wound Staphaureus
Purple gram stain reaction Gram Positive
Patients should avoid aspirin and vitamin C for how long 7 and 3 days
Test performed to differentiate staph organisms Coagulase
Most common bacteria recovered from urine E. coli
Specimen that is inoculated right from the source of infection Direct Culture
Procedure to measure the growth of bacteria in the presence of antibiotics Antibiotic Sensitivity/Susceptibility Testing
Scientific name for the pinworm Enterobius Vermicularis
Test done for the most effective antibiotic Kirby Bauer
Comma or Spiral shaped bacteria Spirilla
Round or oval bacteria Cocci
Rod-shaped bacteria Bacilli
Cocci that appear singly Micrococci
Cocci that appear in pairs Diplococci
Cocci that appear in grapelike clusters Staphylococci
Cocci that appear in chains Streptococci
Pneumonia is caused by what type of bacteria (shape) Cocci
Strep Throat is caused by what type of bacteria (shape) Cocci
Wound Infection is caused by what type of bacteria (shape) Cocci
Food Poisoning is caused by what type of bacteria (shape) Cocci
Toxic Shock Syndrome is caused by what type of bacteria (shape) Cocci
Gonorrhea is caused by what type of bacteria (shape) Cocci
Meningitis is caused by what type of bacteria (shape) Cocci
Bacillus found side by side like boards Palisades
Bacillus attached end to end in chains Streptobacilli
Botulism is caused by what type of bacteria (shape) Bacilli
TB is caused by what type of bacteria (shape) Bacilli
Typhoid fever is caused by what type of bacteria (shape) Bacilli
Diptheria is caused by what type of bacteria (shape) Bacilli
UTI is caused by what type of bacteria (shape) Bacilli
Plague is caused by what type of bacteria (shape) Bacilli
Legionnaires’ is caused by what type of bacteria (shape) Bacilli
Tetanus is caused by what type of bacteria (shape) Bacilli
Gangrene is caused by what type of bacteria (shape) Bacilli
Syphilis is caused by what type of bacteria (shape) Spirillum/Spirochete
Lyme Disease is caused by what type of bacteria (shape) Spirillum/Spirochete
Pyloric Ulcers (H. Pylori) is caused by what type of bacteria (shape) Spirillum/Spirochete
Food poisoning in US is caused by what type of bacteria (shape) Spirillum/Spirochete
Streptococcus is gram positive
Staphylococcus is gram positive
Klebsiella is gram negative
E. coli is gram negative
Gonorrhea is gram negative
Bacteria grow at what temp? 37 Degrees Celsius
Incubate bacteria for how many hours? 24-72 hours
Aerobic Bacteria Require Oxygen
Anaerobic Bacteria Don’t like Oxygen
Aspergillus is a type of fungi
Candida is also known as yeast
Coccidioides Immitis is also known as valley fever
Skin disease caused by fungi Tinea (Ring Worm), Athlete’s Foot, Jock Itch
Fungi causes what type of pneumonia pneumocysitis
4 Mycology Testing Methods Skin scraping/swabs, KOH Prep, Wet Prep, Sabourad’s dextrose agar
Testing methods for virology cell culture, ELISA, Kit tests (flue, herp, rubella)
Giardia lamblia is caused by contaminated water
Plasmodium causes malaria
Pubic lice is called pthirus pubis
Head lice is called pediculus humanus
Common intestinal parasites Tape worm, round worm, hook worm, pin worm
Testing method for intestinal parasites Formalin preserved stool specimen for microscopic exam
Testing method for malaria thin & thick blood smear
Testing method for trich Urine & Vaginal Exam
Contains substances that visibly change as a result of the metabolic activity of certain organisms Indicator (Differential) Media
Types of selective and indicator media EMB, MacConkey’s
CNA is a type of _________ media selective
A culture that is grown from a single colony taken from a direct culture Pure Culture
Swab of affected area is placed on a microscope slide and gram stained Direct Smear
Created by: jruhde