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Bio22lab2quiz
Lab Quiz 2
| Question | Answer |
|---|---|
| Define Mic? | Mic is smaller constration of drug that stops growth of microorganism |
| Define PPNG? | Is a strain of PCN resistant Nerissa Gonorrhea. |
| Define Supper infection? | It destroys good bacteria & bad bacteria. Some of the bacteria develops resistants microbees, and will grow and mutate. |
| What is the number of cases of MIRSA in 2005? | 3,666,600 cases hospitals in the USA. |
| What is MIRSA? | Methicillin Resistant Staphloccocus aureus. Pt's get this when they did not have it before. |
| The classic technique for determining which antibiotics will be effective against a particular microorganism is the? | Kirby-Bauer Sensitivity Test |
| The Gram stan was invented by? What Year? | Hans Christian Gram 1884 |
| How many step are there Gram Stan? | 4 |
| Name the steps? | 1) Primary stain (Crystal Violet) 2) Mordant (iodine) 3) Decolorizing (ETOH) 4) Counterstain (Safranin) |
| What does Gram-Postive look like? Explain Why it is that color? | Purple, the Gram Postive cell wall is thick & composed of many layers of Peptidoglycan, becomes dehydrated, physically shrinks & becomes impermeability. |
| What does Gram Negative look like? | Red(Pink) Gram Negative- cell wall is has only single layer of peptidoglycan surrounded by various types of lipids. |
| Define Mordant? | (Gram Iodine) will make the stain "stick" more tightly to the cell wall. It helps to intensify the ionic chemical bonds among the crystal violet and the bacteria. |
| How long do you do each step for? | ! minute for each step |
| Characteristic or Disease Part1 | Gram Positive or Negative |
| Sensitivity to Penicillin? | Gram Positive-Heelical Rod |
| Sensitivity to Lysozyme? | Gram Positive |
| Lipid A Endotoxin? (Released when bacteria are lysed | Gram Negative |
| Neisseria Gonorrhea | Gram Negative (Dipioccucus Shape) |
| Strep Throat (Streptococcus) | Gram Postive (Coccus Shape) |
| Cholera (Vibiro Cholerae) | Gram Negative (Rod Shaped) |
| Typhoid Fever | Gram Negative (Rod Shape) |
| Tetanus (Closstridiem Tetani) | Gram Positive (Rod Shape) |
| Diphtheria (Coryne Bacteriaem) | Gram Positive (Rod Shape) |
| How could you differentiate between bacterial pneumonia gram + & viral gram - using the gram stain? | bacterial pneumonia gram + (which will stain purple) viral pneumonia gram - (Which will stain colorless) |
| What is colony forming unit & why can it be used to count viable cells in a sample? | Gives you method of counting broth solution that forms colony from a group of bacteria that can be counted as "CFU". They derive from "clump" or chain. |
| Define serial dilution? | It reduces the cell density so that they can be counted when they are plated. |
| What is plate count? | A Plate count is is between 30-300 colonies. |
| If the count is over 300 what do you put? | TMTC |
| Why do you need a new sterile pipette for each step in the serial dilution? | So that there is no containment from dilution to the next dilution.. |
| Why do we never heat or flame the spreader? | Do the heat killing the Microbees, that are on the spreader that is That is why you go from lesser consideration to higher concentration when spreading the micro on the peti dish. If you need clean use ETOH. |
| What is the normal Dilution rate? | 1:99 |
| How many mills of dilution of microbes do you add? How many mills of dilution is there in vial? | 1ml of microbe is add to 9ml of solution to equal 10ml total. |
| How many test tube do you need for the dilution Test? | 5 tubes |
| What is the oxidase test used for? | Used to identify bacteria containing cytochoride oxidases. oxidase Neg- (enterobacteriaceae) oxidase Pos- Pseudomonadacea |
| Define Citrate? | If the medium turns blue, the organism is citrate positive. If there is no color change, the organism is citrate negative. |
| Define Urease? | Pink= Positive (organism hydrolyze yrea. Yellow= Negative |
| Define EMB Plate? | Select/Differential Selective- dyes that inhebit gram+ Differential- lactose fermanteals gram- grow well(non-lactose fermentation) Gram+ lactose fermenters/gram metlic color. |
| Define Mac Plate? | Sel/dif Neg- non ferenters colorless Pos+ Lactose fermenters Pink |
| Define SIM? | Sufide-Indole-Motility Sulfide + Black Indole + Turn Red Motility + growth |
Created by:
zenink
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