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Bacteria - Micro2

Diagnosis

QuestionAnswer
N. meningitidis Gram- diplococci; metabolizes Maltose/Glucose; VPN (or Thayer-Martin media)
L. monocytogenes Gram+ rod; tumbling motility; catalase+; B-hemolytic; grow @ <4 degrees C
M. tuberculosis acid-fast stain (sputum); Ghon complex on CXR; Lowenstein-Jager agar
M. bovis slow-growing; aerobic
M. leprae nerve biopsy (granuloma = tuberculoid); lepromin+ = tuberculoid
K. pneumoniae Gram- rod; mucoid-appearing colonies; lactose fermenter; bloody "currant-jelly" sputum
L. pneumophila Gram- (weakly); Silver stain; charcoal yeast w/ cystein & iron (sputum)
P. aeruginosa Gram- rod; blue/green colonies; GRAPE odor; lactose-; oxidase+
H. influenza Gram- rod; chocolate agar with Factors X (hemin; ST) & V (NAD; LT); Quellung+
B. pertussis Gram- rod; Bordet-Gengou media (or Regan-Lowe w/ charcoal)
C. diphtheria Gram+ rod; looks like "chinese letters"; K+ tellurite (black colonies)
M. pneumoniae No cell wall; fried egg appearance on culturs (Eaton's agar); has CHOLESTEROL
C. psittaci obligate intra; iodine-; Giemsa+; cell wall lacks MurNAc
C. pneumoniae obligate intra; Giemsa+; lacks MurNAc
C. trachomatis obligate intra; iodine+; Giemsa+; cell wall lacks MurNAc; CLEAR urethral discharge
B. cereus Gram+ rod; spore-former; motile
B. anthracis Gram+ rod; non-motile; PROTEIN capsule; widened mediastinum (CXR) if inhaled; squared-ended
N. gonorrhea Gram- diplococci; metabolizes Glucose; VPN (or Thayer-Martin media)
T. pallidum microaerophilic; spirochete (dark field microscopy); VDRL (Abs against cardiolipin --> SLE, RF, leprosy, mono, syphilis) & FTA-ABS (anti-treponemal Abs); endoflagella
B. burgdorferi microaerophilic; spirochete (dark field microscopy); motile
B. recurrentis microaerophilic; culture during fevers (spirochetes --> dark field microscopy); Wright/Giemsa stains
B. hermsii microaerophilic; culture during fevers (spirochetes --> dark field microscopy); Wright/Giemsa stains
Leptospire interrogans aerobic; "ice tong" appearance; spirochetes in CSF (phase 1) & urine (phase 2); anti-leptospiral IgM detected
Y. pestis bubo fluid --> Gram- rods; bipolar stain resemblind SAFETY PIN
Francisella tularensis Gram- rod; chocolate agar w/ cysteine; DTH+ skin test
Brucella spp. Gram- rod; aerobic; facultative intra (blood/bone marrow); brucellergin+ skin test; catalase+; oxidase+
R. prowazekii Giemsa/Weil-Felix + tests; obligate intra
R. typhi obligate intra
R. tsutsugamushi obligate intra
R. rickettsii obligate intra; Weil-Felix+ test
R. akari obligate intra; Weil-Felix+ test
Coxiella burnetii obligate intra; Weil-Felix negative
H. ducreyi vitamin/amino acid enriched agar @ 10% CO2
Gardnerella vaginalis Clue cells (epithelial covered w/ bacterial rods); B-hemolytic
Candida albicans budding yeast on hyphae
T. vaginalis flagellated protozoans; aerotolerant anaerobes
Ehrlichia chaffeensis (HME) obligate intracellular (cytoplasmic vacuoles of hematopoetic cells)
Anaplasma phagocytophilum (HGE) obligate intracellular (cytoplasmic vacuoles of hematopoetic cells)
Bartonella quintana Gram- rod; facultative intracellular
Bartonella bacilliformis Gram- rod; facultative intracellular
Bartonella henselae Gram- rod; facultative intracellular
S. aureus (infections) Gram+ cocci; catalase+; coagulase+; blood cultures = positive
S. aureus (toxin) Gram+ cocci; coagulase+; catalase+; blood cultures = negative
S. epidermidis Gram+ cocci (clusters); catalase+; coagulase-; novobiocin sensitive
S. saprophyticus Gram+ cocci (clusters); catalase+; coagulase-; novobiocin resistant
S. pneumoniae Gram+ diplococci; a-hemolytic; catalse-; optochin sensitive; Quellung+
S. pyogenes (immune) Gram+ cocci; catalase-; B-hemolytic; bacitracin sensitive; ASO+; "Jones criteria"; TEA-COLORED URINE
S. pyogenes (infections) Gram+ cocci; catalase-; B-hemolytic; bacitracin sensitive; ASO+; throat culture
S. pyogenes (toxin) Gram+ cocci; catalase-; B-hemolytic; bacitracin sensitive; ASO+; throat culture
S. agalactiae (GBS) Gram+ cocci; catalase-; B-hemolytic; bacitracin resistant
E. faecalis Gram+ cocci; catalase-; grows in 40% bile & 6.5% NaCl
S. bovis Gram+ cocci; catalase-; grows in 40% bile, but not 6.5% NaCl
S. viridans Gram+ cocci; catalase-; a-hemolytic; optochin resistant; Quellung+
Shigella dysenteriae Gram- rod (stool); non-motile; does NOT produce H2S or ferment lactose
Salmonella typhi Gram- rod (blood); motile; H2S & lactose fermenter
Salmonella enteritidis Gram- rod (blood); motile; H2S & lactose fermenter
ETEC Gram- rod; ferments lactose; EMB (metallic green); MacConkey (purple); B-hemolytic
EHEC/STEC Gram- rod; ferments lactose; EMB (metallic green); MacConkey (purple); B-hemolytic
EPEC Gram- rod; ferments lactose; EMB (metallic green); MacConkey (purple); B-hemolytic
EIEC Gram- rod; ferments lactose; EMB (metallic green); MacConkey (purple); B-hemolytic
E. coli (others) Gram- rod; ferments lactose; EMB (metallic green); MacConkey (purple); B-hemolytic
Y. enterocolitica Gram- rod; motile @ 25C; lactose non-fermenter
Proteus mirabilis Gram- rod; oxidase-; urease+; lactose non-fermenter
H. pylori Gram- rod; curved, spiral-shaped; motile; urea breath test
V. cholera Gram- rod (comma shaped); motile; lactose non-fermenter; oxidase+; glucose fermenter; TCBS agar (flat yellow colonies)
V. parahaemolytica Gram- rod (comma shaped); motile; lactose non-fermenter; oxidase+; glucose fermenter; TCBS agar (flat yellow colonies)
C. jejuni Gram- rod; S-shaped; oxidase+; urease-; grows best @ 42C; microaerophilic
C. botulinum Gram+ rod; anaerobic; motile; spore-former
C. tetani Gram+ rod; anaerobic; motile; spore-former
C. difficile Gram+ rod; anaerobic; motile; spore-former; Toxin B in STOOL
C. perfringens Gram+ rod; anaerobic; non-motile; spore-former
B. fragilis Gram- rod; anaerobe; motile; makes Vit K
Created by: lamsonma
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