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Protein Synthesis
| Term | Definition |
|---|---|
| Central Dogma | DNA -Transcription> RNA -Translation> Proteins |
| DNA contains... | genes (recipes to make proteins) |
| Proteins the essential molecule... | to constructing and running a functional organism |
| The ribosome is responsible for... | assembling proteins |
| DNA can't... | leave the nucleus |
| RNA | intermediary molecule to turn DNA into proteins |
| mRNA | responsible for copying the recipe (gene) to make a protein and transporting it to the ribosome where the protein will be assembled |
| Transcription | process of a sequence of DNA nucleotides being copied into mRNA |
| How does mRNA know where to begin transcribing? | promoter regions (TATA box) section of DNA found at the beginning of a gene sequence. It's job is to get the attention of RNA polymerase |
| RNA polymerase | an enzyme whose job is to transcribe DNA into mRNA |
| Introns... | stay in the nucleus because they don't code for proteins (not transcribed) |
| Exons... | are transcribed, exit the nucleus, and are used to make a protein (expressed) |
| RNA splicing | enzymes cut out introns, so only exons are transcribed |
| Ribosome structure | 1. has a large subunit binded with a small subunit at three different mRNA binding sites (E site, P site, A site) 2. rRNA (ribosomal RNA) makes up about 60% of ribosome structure (40% protein) |
| Ribosomes are essential to... | protein synthesis because ribosomes are the site of protein synthesis |
| The mRNA sequence is read and translated 3 nucleotides at a time. Each section of 3 nucleotides is called a... | codon |
| Anticodon | The complementary tRNA 3 nucleotide sequence |
| Translation | 1. mRNA exits the nucleus and travels to the ribosomes 2. mRNA strand feeds through the ribosome like a dollar bill into a vending machine, entering next to the a site |
| Which two places in the cell can ribosomes be found? | cytoplasm and rough endoplasmic reticulum |
| tRNA | 1. translation RNA 2. decodes the mRNA strand into a sequence of amino acids 3. It is this sequence of amino acids that defines the structure and function of the protein |
| When do mutations occur? | S phase (DNA replication) |
| Types of mutations | 1. Substitution 2. Frameshift 3. Insertion 4. Deletion 5. Point Mutation |
| Substitution | 1 nucleotide (base pair) is substituted for another (can happen if DNA polymerase doesn't catch a mistake when proofreading a new DNA strand |
| Frameshift | mutations that add or remove nucleotides result in a frameshift which is a change is the entire reading sequence after that mutation |
| Insertion | Extra nucleotides are added to sequence |
| Deletion | Nucleotide(s) are removed from sequence during replication |
| Point Mutation | Substitution, Insertion, or deletion of one nucleotide |