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Raymond-Lab1
Microscope lab
| Question | Answer |
|---|---|
| The human eye is _______ to see microorganisms. | unable |
| What are the two major types of microscopes? | compound and electron |
| Which microscope uses light waves from a light bulb? | compound microscope |
| Which microscope uses high energy electrons? | electron microscope |
| What are several types of compound microscopes? | phase contrast brightfield darkfield fluorescent |
| What is the most common type of microscope used by microbiologists? | brightfield |
| Define magnification | the ability to enlarge an object |
| How many magnifying lenses do compound microscopes have? | 2 |
| What are the two magnifying lenses on compound microscopes? | objective ocular |
| What are the four lenses on most brightfield microscopes and their magnification power? | scanning objective (5X) low power objective (10X) high-dry objective (40X) oil immersion objective (100X) |
| Where are the objective lenses attached on the microscope? | on the revolving nosepiece |
| A specimen is magnified first by the ______ lens and then by the ______ lens. | first by the objective then the ocular |
| The total magnification of a specimen is _______ X ________. | Total Mag = Ocular X Objective |
| What two factors determine how well you will be able to see your specimen? | magnification and resolving power |
| What is another word for resolving power? | resolution |
| Define resolution. | Resolution describes how clear you can see your object when viewed through the lenses of your microscope. |
| How is the resolution measured? | How well you can see two lines lying close together as two distinct lines |
| Where can you find the numerical aperture? | written on the side of the lens |
| What does the numerical aperture describe? | How well your lens is able to bend light. |
| What is another name for numerical aperture? | refractive index |
| What two factors affect the resolving power? | numerical aperture and wavelength of light |
| Smaller wavelengths of light produce _______ resolution. | better |
| What is the formula for the resolving power of a microscope? | resolving power = wavelength of light/2 X numerical aperture |
| As the magnification increases, field of view and light __________. | decrease |
| More powerful lenses of a microscope have _______ size lenses. | smaller |
| What does a condenser do? | concentrates the light beam and directs it through the specimen |
| What do the diaphragms of a microscope do? | regulate the intensity of the light entering the lenses |
| What is a rheostat? | Some microscopes have these to also regulate light intensity. |
| The oil immersion objective _____ be immersed into a drop of oil in order for it to work. | must |
| What has the same refractive index as glass? | immersion oil |
| You can/cannot rotate any other objectives through immersion oil. | cannot |
| What does parafocal mean? | when a specimen is in focus with one objective, it will be approximately in focus with the other objectives |
| What is the total magnification you see through the ocular with the oil immersion lens in place? | 1000X |
| As you go from low to high-dry objectives, the diameter of the iris diaphragm should ________. | increase |
| What part of the microscope is directly under your eye when using? | the ocular |
| What part of the microscope must never be turned when the oil immersion objective is in place? | coarse adjustment knob |
| What objective should be in place when storing a microscope? | scanning objective |
Created by:
mastrgurl
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