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Biology 2

Notes for 3rd Mastery

QuestionAnswer
It is an artificially made DNA strand formed by the combination of two or more gene sequences RECOMBINANT DNA
Combining genetic material from different organisms RECOMBINANT DNA
It deals with the technique of identifying, manipulating and transferring of specific genes GENETIC ENGINEERING
GMOs are created by introducing BLANK to organisms by any means RECOMBINANT DNA
What are the important process of rDNA? Isolation of DNA Cutting the DNA restriction enzymes Gene Cloning Ligation Transformation and Transfection
What is the extraction of DNA of interest from donor organisms? Isolation of DNA
This is also known as molecular scissors Restriction enzymes
These things generate stick ends or blunt ends Restriction enzymes
These are overhanging sequences of DNA after being cut by enzymes Sticky Ends
DNA fragment containing gene of interest inserted into a vector Gene Cloning
What are examples of vectors? Plasmids, bacteriophages or viral DNA
the sticky ends of the fragment and vector are joined together using and enzyme called DNA Ligase Ligation
What is the enzyme using to join together the sticky ends and the vector? DNA ligase
Transformation is? The rDNA is introduced into the host nitrogencells (bacteria) while transfection (eukaryotic cells)
It is the technique used to deliver DNA to animal cell Electroporation
A circular self-replicating DNA found in bacterial cell Plasmids
Acts as vectors for gene transfer Plasmids
What are the major applications of DNA? Agricultural application, Medicinal Application, Industrial Application
What are the Agricultural Applications of DNA? Development of Hybridization Development of Transgenic Plants Development of root nodules in cereal plants Development of C4 plants
What are the Medicinal Applications of DNA? Antibiotics Insulin Vaccines Enzymes Interferon Gene Therapy
What are industrial applications of DNA? Chemical compounds improvement Improvement of existing Fermentation
What does this refer to in the application of DNA? Cross parents with different traits Development of Hybridization
What does this refer to in the application of DNA? Contains foreign genes/developed resistance Development of Transgenic Plants
What does this refer to in the application of DNA? Nitrogen fixation bacteria can now be transferred Development of root nodules in cereal plants
What does this refer to in the application of DNA? Improved Photosynthetic efficiency Development of C4 Plants
Two types of Pollination, what are they? Self=Pollination and Cross-Pollination
What type of pollination is from the same flower? Self-Pollination
What type of Pollination is from different flowers? Cross-Pollination
These are the product of self-pollination Pure-line selection
It is a record that depicts the history of the Earth? Geological Time Scale
Created by: annebelle
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