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Enzyme experiments

Application if heat, pH, immobilisation

TermDefinition
Temperature in enzyme activity Factor kept constant Buffer solution
Factors varied Different water bath temperatures
Procedure Add hydrogen peroxide into the graduated cylinder containing the chopped celery buffer solution and washing up liquid. Note the volume, start the stopwatch and record the volume of foam produced in 2 mins.
Results of temperature on enzymes As temperature increases the greater volume of foam produced in 2 mins I.e the rate of enzyme activity increases with temperature
Why did you add washing up liquid? Stops oxygen gas evaporating Helps produce foam
Denaturation by heat Process Same set up as application of temperature experiment with 2 graduated cylinders. Stand in water bath of 25 degrees. Add boiled celery into one and un-boiled celery into the other. Observe a presence or absence of foam in each graduated cylinder.
Results Boiled enzyme: no foam Unboiled enzyme: foam
Created by: EObrienHart
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