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Enzymes
| Question | Answer |
|---|---|
| What is activation energy? | The energy required to start a chemical reaction |
| How do enzymes affect the activation energy of a reaction? | Enzymes decrease activation energy |
| What is the primary role of enzymes in chemical reactions? | To increase the rate of reactions |
| What term describes the specific region of an enzyme where the substrate binds? | Active site |
| What is the purpose of the blank tube in the enzymatic reaction setup? | To control for the enzyme activity |
| Most enzymes are classified as which type of organic compound? | Proteins |
| Why does lemon juice prevent an apple from turning brown? | It lowers the pH and inactivates the enzyme polyphenol oxidase |
| Why do we keep materials on ice during enzyme preparation? | To slow down the enzyme degradation and prevent denaturation |
| In an absorbance vs time graph for an enzymatic reaction, what does increased absorbance indicate? | Increase in product concentration |
| How can you tell when an enzymatic reaction has reached equilibrium? | The absorbance stops increasing |
| What is the supernatant in a centrifuged sample? | The liquid above the pellet |
| What does the pellet represent after centrifugation? | The heavy particles that settle at the bottom |
| What factor does not affect enzyme activity? | Color of the enzyzme |
| What role does a cofactor play in enzyme activity? | It binds to the enzyme and helps in catalysis |
| What happens to enzymes at high temperatures? | They denature and lose their function |
| What is the effect of increasing substrate concentration on enzyme activity, assuming enzyme saturation is not reached? | It increases enzyme activity |
| What type of enzymes typically catalyzes? | Both exothermic and endothermic reactions |
| What is a common method to measure enzyme activity in a lab setting? | Measuring absorbance changes in a solution |
| What is the effect of increasing substrate concentration on enzyme activity, assuming all other factors are constant? | It initially increases enzyme activity until a maximum rate is reached |
| What happens to enzymes at high temperatures? | They are denatured |
Created by:
ortizal126
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