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BIOL1230
Lab Assessment #3
| Question | Answer |
|---|---|
| Why is it necessary to take samples of environmental media? | To identify an infectious agent.To monitor the health status of an ecosystems.To monitor the status of bioremediation activities.Quality Assurance/Quality control activities in the industry and food/beverage production processes.To find new microb |
| Name and describe 2 methods of quantifying microorganisms (include any mathematical calculations involved). | 1. Serial Dilution-a series of 1:10 dilutions in hopes of obtaining a countable number of CFUs. Formula..# of colonies X (1/sample volumn added to plate) X (1/dilution) = # organisms/1mL of smaple2. Most Probable Number (MPN)-uses a series of phenol re |
| Whate does CFU stand for? | Coloy Forming Unit |
| What does an acid and gas positive result look like for the carbohydrate fermentation? | yellow with bubbles in the tube |
| If we were to use Candida albicans for the antibiotic dish difusion test, how would you pridict the results to look and why? | no zone of inhibition because Candida albicans are yeastlike fugus that is not sensitive to bacterial antibiotics |
| What media did we used for the Kirby-Bauer tests? | MHA - Mueller Hinton Agar |
| Is there a way to determine if resistance is developing within a microbial population based on your antibiotic disk diffusion results? | If you observe colonies within the zone of inhibition. |
| What is a Plasmid? | self-replicating extrachromosomal, double-stranded cirular DNA molecules found in many strain of bacteria. |
| What genes were included in the plasmid vector used for transforming E. Coli? | a gfp gene called PFluoroGreen and a antibiotic resistance gene |
| What was the purpose of the (-) tube in the transformation procedure? | act as experimental control |
| What was the purpose of the heat-shock step in the transformation procedure, and at what temperature was it carried out? | Make the memebrane more premable at 42 degrees C |
| What is the approximate efficiency rate of the heat-shock transformation procedure? | 1/10,000 |
| Name 3 classes of helminths. Are they prokaryotes or eukaroytes? | Multicellular eukaryotic organisms - Nematodes, Trematoda, & Cestoda |
| Name and define the two stages of the protozoan life cycle. | Trophozoite - feeding and growing stageCyst - resistant stage |
| Which protozoan phylum is nonmotile? | Apicomplexa |
| Name two protozoan diseases and the causative agent for each. | Malaria - plasmodium sppToxoplasmosis - Toxoplasma gondii |
| Why are helminths classified among mircoorganisms? | At one point in their life, they were a microorganism -larva, etc |
| What was the purpose of the 30-minute incubation in Luria broth at 37 degree C? | allow the cells to recover from the heat shock step before we plate them in the hostile environment of the antibiotic plate |
| Why do we grow transformed cells on antibiotic plates? Is it necessary to keep transfromed cells in an antibiotice aft erh initial plating? Why nor why nOt? | to slect against any cell that was not transformed....yes...this provents cells who do not inherit the plasmid from taking over your culture |
| Why is the target gene cloned under the lac operon? What mechanism is used to control expression? | we can control expression so that the target gene is overexpressedwhile the culture is in the exponential growth phase...ensures we can harvest large quantities of the target protein for experimentation...Lactos |
Created by:
yinboyer
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