click below
click below
Normal Size Small Size show me how
Biol Ch. 16
DNA Replication
| Term | Definition |
|---|---|
| The experiments of Meselson and Stahl showed that DNA | replicates in a semiconservative fashion |
| The unwinding of DNA at the replication fork causes twisting and strain in the DNA ahead of the fork, which is relieved by an enzyme called | topoisomerase |
| Enzyme that links Okazaki fragments together on the lagging strand | Ligase |
| Proteins that are involved in packaging the eukaryotic chromosome into “beads” called _ are _ | nucleosomes; histones |
| Telomeres are | repeating noncoding sequences at the ends of linear eukaryotic chromosomes |
| In DNA replication, helicases | separate the two strands of the double helix |
| In DNA replication, DNA polymerases | construct two new strands using each of the original strands as templates |
| Griffith showed that dead Pathogenic cells can | transform living nonpathogenuc cells into living pathogenic cells |
| The role of DNA polymerases in DNA replication is to | attach free nucleotides to the new DNA strand |
| Of the three alternative models of DNA replication, which are the __________ models, the one that explains how DNA replicates is the __________ model | conservative, semiconservative, and dispersive; semiconservative |
| Radioactive sulfur was used to label _ | Protein |
| Radioactive phosphorus was used to label _ | DNA |
| Hershey and Chase Experiment proved that | the genetic material of a bacteriophage is DNA |
| DNA is always synthesized from _ to _ | 5' to 3' |
| DNA strands are | Directional Complementary Antiparallel |
| The protein Helicase | Binds to the origin Breaks the hydrogen bonds Creates a replication bubble with replication forks |
| Replication forks | sites of DNA replication where the replisome is formed |
| The replisome has | all the necessary enzymes to replicate DNA |
| Helicase function | continuously unzips DNA for replication |
| Single-stranded binding proteins (SSBs) | Keep the DNA strands separate by capping exposed nucleotides |
| Topoisomerase | Keeps DNA from snapping back together after it's unwound by relieving tension |
| DNA Polymerase III | synthesizes new DNA using the original strand as a template |
| DNA Primase | creates short (5-10 nucleotides) RNA primers for DNA Polymerase III to build off of |
| DNA Polymerase III requires a | starting 3'-OH to attach to, it can't make a chain from scratch and free nucleotides as building materials along with energy |
| The starting 3'-OH is found on | the primer |
| One DNA strand is _ and the other is _ | made continuously -> Leading strand made in fragments -> Lagging strand |
| Leading strand | synthesized continuously |
| Lagging strand | synthesized in fragments |
| DNA ligase | joins Okazaki fragments |
| Okazaki fragments | the DNA fragments of the lagging strand |
| Griffith experiment | Bacteria transformation |
| Hershey and Chase experiment | Bacteriophage; discovered DNA was genetic material through viruses and e. coli (Radioactive sulfur and phosphorus) |
| Conservative replication | Original DNA remains untouched |
| Meselson and Stahl experiment | Discovered semiconservative DNA replication through N^15 and N^14 |