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Biol Ch. 16

DNA Replication

TermDefinition
The experiments of Meselson and Stahl showed that DNA replicates in a semiconservative fashion
The unwinding of DNA at the replication fork causes twisting and strain in the DNA ahead of the fork, which is relieved by an enzyme called topoisomerase
Enzyme that links Okazaki fragments together on the lagging strand Ligase
Proteins that are involved in packaging the eukaryotic chromosome into “beads” called _ are _ nucleosomes; histones
Telomeres are repeating noncoding sequences at the ends of linear eukaryotic chromosomes
In DNA replication, helicases separate the two strands of the double helix
In DNA replication, DNA polymerases construct two new strands using each of the original strands as templates
Griffith showed that dead Pathogenic cells can transform living nonpathogenuc cells into living pathogenic cells
The role of DNA polymerases in DNA replication is to attach free nucleotides to the new DNA strand
Of the three alternative models of DNA replication, which are the __________ models, the one that explains how DNA replicates is the __________ model conservative, semiconservative, and dispersive; semiconservative
Radioactive sulfur was used to label _ Protein
Radioactive phosphorus was used to label _ DNA
Hershey and Chase Experiment proved that the genetic material of a bacteriophage is DNA
DNA is always synthesized from _ to _ 5' to 3'
DNA strands are Directional Complementary Antiparallel
The protein Helicase Binds to the origin Breaks the hydrogen bonds Creates a replication bubble with replication forks
Replication forks sites of DNA replication where the replisome is formed
The replisome has all the necessary enzymes to replicate DNA
Helicase function continuously unzips DNA for replication
Single-stranded binding proteins (SSBs) Keep the DNA strands separate by capping exposed nucleotides
Topoisomerase Keeps DNA from snapping back together after it's unwound by relieving tension
DNA Polymerase III synthesizes new DNA using the original strand as a template
DNA Primase creates short (5-10 nucleotides) RNA primers for DNA Polymerase III to build off of
DNA Polymerase III requires a starting 3'-OH to attach to, it can't make a chain from scratch and free nucleotides as building materials along with energy
The starting 3'-OH is found on the primer
One DNA strand is _ and the other is _ made continuously -> Leading strand made in fragments -> Lagging strand
Leading strand synthesized continuously
Lagging strand synthesized in fragments
DNA ligase joins Okazaki fragments
Okazaki fragments the DNA fragments of the lagging strand
Griffith experiment Bacteria transformation
Hershey and Chase experiment Bacteriophage; discovered DNA was genetic material through viruses and e. coli (Radioactive sulfur and phosphorus)
Conservative replication Original DNA remains untouched
Meselson and Stahl experiment Discovered semiconservative DNA replication through N^15 and N^14
Created by: 1763
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