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Microbio Lab 1 Vocab
Microbio Lab 1
| Question | Answer |
|---|---|
| Inoculation | Implantation of microbes into or upon culture media |
| Inoculum | The cells introduced/ used in inoculation or to add to a culture |
| Medium | A nutrient used to grow organisms outside their natural habitats |
| Culture | Visible accumulation of microbes in or on a nutrient medium |
| Pure Culture | A container growing a single species of microbes whos identity is unknown |
| Mixed Culture | A container growing two or more different, known species of microbes |
| Contaminated Culture | A culture in which bacteria from a foreign source have infiltrated the growth medium |
| Axenic Culture | Pure cultures, sterile state, free of contamination while transferring living organisms |
| Incubator | Instrument used to encourage microbial growth. Isolates a sample culture in a temperature-controlled environment |
| Isolation | The separation of microbial cells on solid media to create discreet colonies |
| Macroscopic inspection | Visible to the naked eye. To view a sample with low magnification |
| Microscopic inspection | Invisible to the naked eye. Use a microscope |
| Simple stains | Application of a single staining compound (dye) to a smear of heat-fixed bacteria. |
| Differential stain | Application of two or more reagents capable of imparting color to a cell or it's parts. Gam stain is commonly used |
| Primary dye | Crystal violet. Gram pos and neg affix to this dye |
| Counterstain | Safranin. Makes gram neg bacterial cells visible. Applied after decolorization |
| Ocular | Lens is in the upper portion of the body tube. 10X magnification and has a pointer inside. |
| Revolving nosepiece | Used for different magnifications. has three or more objectives attached. 10X, 40X, 100X |
| Low-power objective | 10X magnification |
| High-dry objective | 40X magnification |
| Oil immersion objective | 100X magnification |
| Arm | Connects the base to the body tube |
| Fine adjustment | Smaller knob that helps bring the specimen into focus |
| Coarse adjustment | Raises or lowers stage |
| Stage | Lies below objectives and holds the microscope slide for viewing |
| Stage clips | Holds the slide in place |
| Stage adjustments | Used to move slide during viewing |
| Condenser lens | Lens is substage and serves to concentrate and align light rays before the pass through a specimen |
| Iris diaphragm | Attached to the condenser. Lever that adjusts the amount of light passing through a specimen |
| Iluminator | Found in the base beneath the stage |
| Stage | Portion that rests on the table |
| Plates | Petri dishes that hold 25mL of sterilized agar medium. |
| Slants | Tubes that hold 10 mL of liquid medium/ Contain solid agar that solidifies at an angle/ provides a greater surface area/ capped and sterilized |
| Deeps | Tubes that hold 10 mL of solidified agar and is in the upright position/ Capped and sterilized |
| Nutrient Broth | Liquid that contains nutrients required for microbial growth |
Created by:
Clinton Perdue
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