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Biology

PRACTICAL: Investigating enzyme activity

QuestionAnswer
What 12 pieces of apparatus are needed for this experiment? 1. Spotting tile 2. Measuring cylinder 3. Test tube 4. Syringe 5. Pipette 6. Stopwatch 7. Water 8. Thermometer 9. Water bath 10. Iodine 11. Starch solution 12. Amylase solution
What liquid do we put in each well of the spotting tile? Iodine
How do we heat the starch solution and amylase solution before mixing them? Using a water bath
How long should we take a between putting a drop of the solution into an iodine well? 1 minute
How can we see when the amylase has broken down all the starch? The solution stops turning blue-black and stays orange
Give a suitable range of temperatures to conduct this experiment with 20 - 60 degrees
What are the results of the experiment? 1. Took a long time to stop changing colour at colder temperatures 2. Iodine stopped changing colour fastest at optimum temperature 3. Iodine didn't stop changing colour at hotter temperatures
What does this experiment prove? The rate of enzyme activity is fastest at optimum temperature and the enzymes denature and cannot work if temperature becomes too hot
What are we changing in this experiment? The temperature for each repeat
What are 2 things are we controlling in this experiment? 1. The volume of starch/amylase/iodine 2. The concentration of starch/amylase/iodine
Created by: JoeMather
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