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CET - Cell Structure

Cells, Exchange and Transport, OCR A level Biology F211

QuestionAnswer
Magnification? Increases size of image, does not increase detail level.
Resolution? Ability to see two distinct points separately; the higher the resolution the more detail one can see.
Why stain? Image shows on microscope because different amounts of light or electrons are absorbed, but sometimes a specimen lets light or electrons pass straight through.
How do you stain for a light microscope? Dye added, taken in better by some parts than others, cause a contrast.
How do you stain for an electron microscope? Object dipped in solution of heavy metals which is taken in better by some parts than others. Metal ions scatter electrons, creating a contrast.
What is the maximum resolution and magnification of a light microscope? 200nm, x1500.
Maximum resolution and magnification of transmission electron microscope? 0.1nm, over x1,000,000.
Maximum resolution and maginification of scanning electron microscope? 5nm, less then 1,000,000.
Describe the plasma membrane? Membrane found on surface of animal cells and just inside cell wall of plant cells and prokaryotic cells; made of lipids and protein.
WWhat is the function of the plasma membrane? Regulates movement of substances into and out of cell. Has receptors which allow it to respond to chemicals like hormones.
Describe the cell wall? Rigid structure that surrounds plant cells, made mostly of the carbohydrate cellulose.
What is the function of the cell wall? Supports plant cells.
Describe the nucleus? Large, surrounded by nuclear envelope (double membrane) containing many pores. Contains chromatin, and often a nucleolus.
What is the function of the nucleus? Chromatin made from proteins and DNA, which controls cell activity. Pores allow substances to move between nucleus and cytoplasm. Nucleolus makes ribosomes.
Describe a lysosome? Round organelle surrounded by a membrane; no clear internal structure.
What is the function of an lysosome? Contains digestive enzymes; used to digest invading cells or break down worn components of cell.
Describe a ribosome? Very small, either floats free in cytoplasm or attached to rough ER.
What is the function of a ribosome? Site where proteins are made.
Describe rough endoplasmic reticulum? System of membranes enclosing a fluid filled space; surface is covered with ribosomes.
What is the function of rough endoplasmic reticulum? Folds and processes proteins that have been made at the ribosomes.
Describe smooth endoplasmic reticulum? Similar to rough ER but no ribosomes.
What is the function of smooth endoplasmic reticulum? Synthesises and processes lipids.
Describe a vesicle? Small, fluid filled sac in the cytoplasm, surrounded by a membrane.
What is the function of a vesicle? Transports substances in and out of the cell and between organelles. Some formed by Golgi apparatus or ER, others at cell surface.
Describe Golgi apparatus? Group of fluid filled flattened sacs, vesicles often seen at edges of sacs.
What is the function of Golgi apparatus? Processes and packages new lipids and proteins; also makes lysosomes.
Describe mitochondria? Usually oval shaped. Double membrane, inner folded forming cristae, inside is matrix, contains enzymes involved in respiration.
What is the function of mitochondria? Site of aerobic respiration, where ATP is produced. Found in large numbers in cells which require a lot of energy.
Describe a chloroplast? Small, flattened structure in plant cells. Surrounded by double membrane; has membranes inside called thylakoid membranes which are stacked up in some parts to form grana, which are linked by lamellae - thin, flat pieces of thylakoid membranes.
What is the function of a chloroplast? Site of photosynthesis. Some parts happen in the grana, and others in the stroma, a thick fluid found in chloroplasts.
Describe a centriole? Small, hollow cylinders, containing a ring of microtubules.
What is the function of a centriole? Involved with separation of chromosomes during cell division.
Describe cilia? Small, hair like structures on surface membrane of some animal cells. In cross section have outer membrane and ring of nine pairs of microtubules inside, with a single pair of microtubules in the middle.
What is the function of cilia? Microtubules allow to move, used by cell to move substances along cell surface.
Describe the flagellum? On Eukaryotic cells - like cilia but longer. Stick out from the surface and are surrounded by plasma membrane. A pair of microtubules surrounded by nine pairs around the edge.
What is the function of a flagellum? Microtubules contract to make flagellum move. Flagella used to propel cell forward.
Where are different types of protein made? Ribosomes on rough ER make proteins that are excreted or stay attached to the cell membrane. Free ribosomes make proteins that stay in the cytoplasm.
Where are proteins produced at rough ER folded and processed? In the rough ER.
What are proteins transported from rough ER to Goldi apparatus in? Vesicles.
What happens at the Golgi apparatus? Proteins undergo further processing.
What happens after proteins are processed in the Golgi apparatus? Enter more vesicles to be transported around cell.
How are the protein threads of the cytoskeleton arranged in eukaryotic cells? As microfilaments (small solid strands) and microtubules (tiny protein cylinders).
What are the four main functions of the cytoskeleton? 1. Support the cells organelles. 2. Strengthen cell / maintain its shape. 3. Transporting materials within cell. 4. Causes cell to move.
What is the diameter of a prokaryotic and eukaryotic cell? Prokaryotic: less then 2um. Eukaryotic: 2-200um.
Difference in DNA between prokaryotic and eukaryotic? Prokaryotic: Circular. Eukaryotic: Linear.
Difference in nucleus between prokaryotic and eukaryotic? Prokaryotic: No nucleus; DNA free in cytoplasm. Eukaryotic: Nucleus present; DNA inside nucleus.
Cell wall? Prokaryotic and eukaryotic. Prokaryotic: Murein cell wall. Eukaryotic: None in animals; cellulose in plants; chitin in fungi.
Number of organelles? Prokaryotic vs. eukaryotic. Prokaryotic: Few organelles, no mitochondria. Eukaryotic: Lots of organelles, mitochondria present.
Ribosome size in prokaryotic vs. eukaryotic? Prokaryotic: Smaller. Eukaryotic: Larger.
Size difference? Prokaryotic vs. eukaryotic. Prokaryotic roughly 1/10 size of eukaryotic, so need to be viewed through electron microscoope.
What do plant cells have which animal cells don't? 1. Cell wall with plasmodesmata ('holes' for substance exchange). 2. Vacuole (compartment containing cell sap). 3. Chloroplasts for photosynthesis.
Created by: emm142
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