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Exercises 4-6
Learning Objectives 4-6
| Question | Answer |
|---|---|
| What is the difference between defined and complex media and when are they used? | Difference is the amounts of each particular nutrient may vary from recipe to recipe. |
| Compare and contrast the the 4 forms of agar medium? | Agar Slant- Liquefied medium solidified at a slanted angle Agar Deep- Liquefied medium solidified upright Agar Pour- Liquefied medium waiting to be poured later Agar Plate-Liquefied medium being poured into Petri plate |
| What are the methods of sterilization? | Autoclave- sterilizes media and instruments (quick) Dry Heat Sterilization- sterilizes glassware Filtration- sterilizes liquids Ultraviolet Radiation- sterilizes air and surfaces (hospitals use) |
| What is contamination? | It is when something in the lab is not pure and clean from microbes. Contamination can occur through air + inanimate objects |
| What is aseptic technique? | A process used to preserve the purity of objects. Through the use of Bunsen Burners |
| How is a bunsen burner used? | It is a flame that is used to kill any organisms that may be present and will sterilize the object |
| Compare and contrast the methods of aseptically transferring orgs. from different forms of media? | Inoculation- removed from one medium using inoculating loop and is transferred to a fresh medium ; also known as subculturing |
| What is the purpose of making a smear and what is the procedure? | This to allow the sample to be visible under the microscope because they appear colorless. 1. Solid culture is mixed with a drop of water and spread on glass 2. Let it air dry 3. |
| What are some errors that can happen during smear prep and what some solutions? | 1. Too much water, too much or too little organisms used, overmixing are some errors. 2. Let it air dry all the way |
| Difference between simple stains and differential stains? | Simple stains only use one dye (cell size, shape, arrangement) Differential stains use two or more dyes (cellular components) |
| Compare and contrast cell walls of Gram positive and gram negative | Gram positive cell wall: composed of thick layer of peptidoglycan, contain teichoic acids and lipoteichoic Gram negative cell wall: thin layer of peptidoglycan, contain lipopolysaccharides (Lipid A) |
| What are the 4 steps of the Gram stain procedure? | 1. (Primary stain) Heat fixed smear is stained with crystal violet 2. (Mordant) gram iodine is added 3. Cells are washed with decolorizer (95% ethanol) 4. (Counterstain) the bacteria with safrain |
| What are possible errors that can occur during gram staining? | Performing on OLD bacteria |
Created by:
Fatou18
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