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Micro Lab 1-3
Learning Objectives 1-3
| Question | Answer |
|---|---|
| What is a microorganism? | An organism only visible with a microscope |
| Difference between 3 domains of life? | Eukarya have a distinct membrane bound nucleus; Archaea and Bacteria do NOT have a nucleus. |
| What is the purpose of microscope? | To view microorganisms |
| What are the different parts of the microscope? | Objective Lens (10X, 40X, 100X)- above the specimen Ocular Lens- where you view the specimen High power objective - fine adjustment knob Low power objective - course adjustment knob 100X must use immersion oil |
| How to calculate total magnification? | Objective magnification x ocular magnification |
| Difference between magnification and resolving power? | Resolving power is being able to distinguish between two objects as separate entities; Magnification is combination of magnification of ocular and objective lens |
| Different types of microscopes | Dark field- unstained orgs Phase Contrast-internal structures Fluorescence- medical diagnosis Electron Microscope- TEM + SEM |
| Correct transportation of microscope | Hold arm with one hand and base with the other hand |
| What is Endosymbiotic Theory? | Helps explain why prokaryotic characteristics are found in eukaryotic cells |
| Why are fungi important for decomposition and nutrient cycle? | They break down dead and decaying orgs. which is essential to earth's nutrients cycle. They put the nutrients they decompose back into the environment |
| What is the symbiotic relationship between fungi and plants | Fungi provide mineralized nutrients to plants in exchange for continous supply of carbs and other compound |
| What are structures found in fungi and explain their function? | Hyphae used for nutrient absorption Collective mass of hypae are mycelium; seen on foods |
| Difference in reproduction in mold and yeasts | Molds (multicellular) reproduce asexually through spores Yeast (unicellular) reproduce asexually through budding |
| Discuss dimorphism | Means that some fungi have two different growth forms Mold grows at room temp. Yeast grow at human body temp. |
| Properties of Sabouraud's Dextrose Agar | Has high sugar concentration and slightly acidic which makes it unsuitable for bacteria to grow. (used to fungi) |
| What is lactophenol cotton blue stain? | It is used to view fungal specimens because it kills and preserves the organism. |
| What are the pros and cons of algae? | Pro: they produce majority of the world's oxygen (primary producer) Cons: produce toxins that cause problems for humans |
| Differentiate between groups of parasitic worms? | Flatworms: platyhelminthes Roundworms:Nematoda Tapeworms: Cestodes (beef lamb, pork) (most common) |
| What is the infective stage of malaria? | Sporozoites- formed by sexual reproduction within the mosquitos. |