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Bio Chapter 7
DNA Structure and Gene Function
| Question | Answer |
|---|---|
| Mutation creates slightly different versions of the same genes, called ______. | alleles |
| If a template strand of DNA is: 3' T A G C A T 5' What will the strand of RNA made during transcription be? | 5' AUCGUA 3' |
| During translation, tRNA transfer ____ to build a protein. | Amino Acids |
| Which protein/enzyme is responsible for making RNA using a DNA template? | RNA Polymerase |
| In a frameshift mutation, all of the amino acids before the shift are changed. (True or False) | False |
| RNA polymerase binds to the promoter to start transcription. (True or False) | True |
| This gene sequence undergoes ____________ (i.e., is copied) into ____________ by RNA polymerase and other proteins. This occurs in the ____________ of the cell. | transcription, ,nucleus |
| Reading from 5' to 3', this RNA product has the sequence AUUGCGGCAAGCAGU . It binds to the small subunit of the ____________ . It is joined by the large subunit, completing the ____________ stage of the process of ____________ . | ribosome, elongation, translation |
| Each mRNA 3-base sequence, called a ____________ , matches with a tRNA sequence called the ____________ . The tRNA also carries the specified ____________ , used as a building block for a protein. | codon, anticodon, amino acid, |
| Select all the statements about nucleotides that are accurate. | -DNA always contains the same sugar in its nucleotides. -They are composed of a sugar, a nitrogen-containing base, and one or more phosphorus-containing groups. |
| Select all of the following that are true about codons. | They are composed of three mRNA bases. They correspond to one amino acid. |
| In the Hershey and Chase experiment, ______ was found inside the infected bacteria, indicating that the labeled viral ______ had entered the bacterial cells to direct new virus production. | radioactive phosphorus; DNA |
| In his experiments with S. pneumoniae bacteria and mice, Frederich Griffith found that | something in heat-killed S bacteria could transform type R bacteria. |
| In an RNA molecule, a(n) ______ is a genetic "code word" that corresponds to one amino acid. | Codon |
| Hershey and Chase showed that the part of a virus that could enter bacterial cells and cause the production of more viruses was | DNA |
| Select all of the correct statements regarding the results of Frederick Griffith's 1928 experiment involving Streptococcus pneumoniae. | Live S strains caused pneumonia in mice. Heat-killed S strains mixed with live R strains caused pneumonia in mice. |
| Subunits called ______ are the building blocks of nucleic acids, as determined by scientists by about 1930. | Nucleotides |
| The promoter is a sequence on the ______ that signals the start of a gene. | Template stand |
| In eukaryotes, a part of an mRNA molecule that is removed before translation is called a(n) | Intron |
| In the Hershey and Chase experiment, radioactively-labeled sulfur was found in the ______ within one test tube, while radioactively-labeled phosphorus was found in the ______ within the other test tube, indicating that ______ was the genetic material. | fluid; bacterial cells; DNA |
| At the beginning of transcription, RNA polymerase binds to a ________. | promoter |
| In transcription, the genetic information in DNA is copied into a(n) ______ molecule, and in translation, the "message" in an mRNA molecule is translated into a(n) ______ sequence. | mRNA; amino acid |
| On a tRNA molecule, the __________ is a three-base sequence that is complementary to and binds to an mRNA codon during translation. | anticodon |
| Complete this sentence: If the nucleotides ATT were inserted into the template strand of a gene, then | the result protein will be shorter and nonfunctional |
| Combines the proteins to form a ribosome | rRNA |
| Carries amino acids to ribosomes and binds to codons | tRNA |
| Its codons specify the order of amino acids in translation | mRNA |
| Consider the types of mutations and the circumstances in which mutations arise to label the TRUE statements below. (Select all that apply.) | -Mutagens and meiosis are two sources of mutation -A single-base insertion is usually more harmful than a single-base substitution -Mutations can be useful to the organism |
| Arrange the following parts and processes of eukaryotic gene expression in chronological order | 1. RNA polymerase binds to gene. 2. Transcription elongation occurs. 3. A complete mRNA strand detaches from RNA polymerase. 4. Introns are removed. 5. Translation occurs. 6. Functional protein is formed |
| Transcription includes: | synthesis of RNA, Nucleotides are joined together, occurs in the nucleus, DNA is the template, requires RNA polymerase |
| Translation Includes: | synthesis of polypeptides, amino acids are joined together, occurs on a ribosome, occurs after RNA processing |
| What does Transcription and Translation both include? | required in order to produce proteins, occurs before protein folding takes place |
| What is the DNA to protein sequence? | 1. RNA polymerase uses DNA template to create complementary strand of mRNA 2. mRNA leaves nucleus 3. tRNA molecules transport amino acids to rib 4. Polypeptides are folded-form proteins 5. Proteins are brought to location- carry out their functions |
| Hydrogen bonds connect _____________ DNA strands | Complementary |
| Transcription occurs in the ______- | Nucleus |
| What are the three steps of Transcription? | initiation, elongation, termination |
| Both the the promoter and operator control _____ ____________. | Gene Expression |
| Point Mutation | Mutation that changes one or a few base pairs in a gene |
| The genetic code shows which | mRNA codons correspond to which amino acids |
| In bacteria, a(n) ________ is a group of genes under control of a single promoter. | Operon |
| Select all of the following that are methods of gene regulation found in eukaryotes but not prokaryotes. | - Splicing exons in alternative ways - Adding methyl groups to silence genes - blocking mRNA exit from the nucleus |
| What are coding regions of eukaryotic mRNA that are translated? | Exons |
| Put the steps in order that they occur during initiation of translation, beginning with the first step at the top | 1. the "cap" end of the mRNA bonds with a small ribosomal subunit 2. A tRNA carrying the amino acid methionine binds to the AUG start codon. 3. The large ribosomal subunit attaches to the small ribosomal subunit |
| In bacterial operons, a DNA sequence that exists between the promoter and the protein coding region is called the _______ and is where a repressor protein can bind | Operator |
| select all of the following that are true about the genetic code. -AUG is usually the last codon in a strand of mRNA -each amino acid is specified by only one codon -three codons are "stop" codons -AUG encodes methionine and "start" | -three codons are "stop codons" -AUG encodes methionine and "start" |
| What is a discrete package of DNA copied around proteins? | Chromosome |
| in eukaryotes, RNA polymerase cannot bind to the promoter and initiate transcription without the presence of regulatory proteins called ______. | Transcription Factors |
| Select all the ways that cells can make DNA less available for transcription -tag DNA with methyl groups -wind of fold DNA in a way that makes in unavailable -unwind DN -remove introns and split exons together | -tag DNA with methyl groups -wind or fold DNA in a way that makes in unavailable |
| In eukaryotic cells, the genome is usually divided up into multiple ___________ | Chromosomes |
| In prokaryotic cells, the genome usually consists of just ____ circular DNA molecule | One |
| epigenetic is he study of ________ that affect gene expression but do not change the DNA sequence | DNA Modifications |
| Viral enzyme that synthesizes DNA from an RNA template | Reverse Transcriptase |
| Ribosmes consist of... | One large and one small subunit |