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micro test 4
gram positive bacilli/neisseria wk sheet
| Question | Answer |
|---|---|
| what are diphtheroids? | nonpathogenic corynebacterium. genus C.... gram pos bacilli,pleomorphic, chinese letter arrangement. |
| where are diphtheroids normal flora? | skin,resp tract/throat,vagina,soil,water. |
| what is the characteristic lesion of C.diphtheriae? | patogen diphtheria. pseudomembrane leathery gray web.. |
| what diseases does the DPT immunize against? | diphtheria,pertussis/whopping cough,tetanus/given at the time of the accident. |
| what is the gram stain of Bacillus? | gram pos. bacilli with spores. |
| is Bacillus an aerobic or anaerobic genus? | aerobic. |
| what is the characteristic lesion of anaerobic infections? | necrosis(dead tissue) |
| which specimens are likely to contain clinically significant anaerobes? | material aspirated with needle + syringe and puss from a deep wound with foul odor. use an anaerobic specimen collection tube. |
| which specimens are unacceptable for anaerobic culture? | A.)swabs from superficial/surface wounds,throat,nose and vagina. B.)expectorated sputum,gastric contents. |
| what is the gram stain of Clostridium? | gram pos. bacilli with spores. |
| are Clostridium aerobic or anaerobic organisms? | anaerobic. |
| where are Clostridium normally found? | soil,water,dust and intestines of humans & animals. |
| 13.describe the disease caused by each of the following and the role of exotoxin in the disease. | A)CI.tetani 1-tetanus/lock jaw.picked up from soil in a puncture wound.2-role of exotoxin-neurotoxin that causes painful muscle spasims.3-characteristics of a wound likely to become infected-deep puncture,burns,umbilical stump. spores or dirt in wound. |
| B.)CI.perfringens 1-can cause gas gangrene. orgs ferments muscle sugars. produces gas that tears tissue apart.2-rose of exotoxin-causes swelling and tissue death. | C.)CI.difficle.1-antiobiotic associated pseudomembranous colitis=diarrhea. |
| what is the gram stain of Neisseria? | gram neg(red) diplococci coffee bean shaped. |
| where are Neisseria species normal flora? | throat & sputumn |
| what does a direct smear of urethral discharge that is positive for gonorrhea look like? | intracellular and/or extracellular gram neg diplococci |
| why should the smear be thin? | necessary to get a accurate gram stain. |
| why is it difficult to interpret the smears from women? | women have normal flora that resembles neisseria. |
| Modified Thayer Martin agar is designed to facilitate the recovery of which organisms? | A.)N.gonorrhoeae=Common name-gonoccus. B.)N.meningitidis=meningococcus. |
| what does MTM use to select organisms? | antibiotics. |
| how should MTM media be inoculated? | Z and then cross streak. |
| what must neisseria have in the atmosphere to grow? | Co2 carbon dioxide . enriched media 24-48 hrs. 37 Celsius. |
| Give the name and a description of the disease cause by neisseria gonorrhoeae? | Gonorrhea-STD/STI STd with purulent discharge . cervix in women ,urethra in men. occasionally found in the rectum,pharynx,and eyes.infects mucous membranes. |
| how is gonorrheal conjunctivitis in newborns prevented? | Ophthalmia neonodorum. credemethod-eyes of newborn rinsed with silver nitrate .required by law. |
| give the name and a description of the disease caused by N.meningitidis? | |
| what is the gram stain of Neisseria? | gram neg(red) diplococci coffee bean shaped. |
| where are Neisseria species normal flora? | throat & sputumn |
| what does a direct smear of urethral discharge that is positive for gonorrhea look like? | intracellular and/or extracellular gram neg diplococci |
| why should the smear be thin? | necessary to get a accurate gram stain. |
| why is it difficult to interpret the smears from women? | women have normal flora that resembles neisseria. |
| Modified Thayer Martin agar is designed to facilitate the recovery of which organisms? | A.)N.gonorrhoeae=Common name-gonoccus. B.)N.meningitidis=meningococcus. |
| what does MTM use to select organisms? | antibiotics. |
| how should MTM media be inoculated? | Z and then cross streak. |
| what must neisseria have in the atmosphere to grow? | Co2 carbon dioxide . enriched media 24-48 hrs. 37 Celsius. |
| Give the name and a description of the disease cause by neisseria gonorrhoeae? | Gonorrhea-STD/STI STd with purulent discharge . cervix in women ,urethra in men. occasionally found in the rectum,pharynx,and eyes.infects mucous membranes. |
| how is gonorrheal conjunctivitis in newborns prevented? | Ophthalmia neonodorum. credemethod-eyes of newborn rinsed with silver nitrate .required by law. |
| give the name and a description of the disease caused by N.meningitidis? | epademic meningitis-purulent inflammation of the meninges)membrane that covers brain and spinal cord, life threatening death in 24-48 hrs symptoms mimic flu/colds |