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Micro - Ch 3
| Question | Answer |
|---|---|
| Compound light microscope | Has a series of lenses and uses visible light as its source of illumination. |
| Ocular | The eyepiece of a microscope where images of a specimen are magnified. |
| Objective lenses | The lenses closest to the specimen |
| Total magnification | Objective lens magnification X ocular lens magnification |
| Resolution | Is the ability of the lenses to distinguish fine detail and structure. |
| Staining | coloring the microorganisms with a dye that emphasizes certain structures. |
| Fixed | attached to the microscope slide. kills and fixes the microorganisms to the slide. |
| Smear | A thin film of material containing the microorganisms that is spread over the surface of the slide. |
| Basic Dyes | A salt in which the color is in the positive ion; used for bacterial stains. |
| Acidic dyes | A salt in which the color is in the negative ion; used for negative staining |
| Simple stain | A method of staining microorganisms with a single basic dye |
| Mordant | A substance added to a staining solution to make it stain more intensely. |
| Gram Stain | A differential stain that classifies bacteria into two groups, gram-pos and gram-neg |
| Differential stains | A stain that distinguishes objects on the basis of reactions to the staining procedure. |
| Gram negative | Bacteria that lose the crystal violet color after decolorizing by alcohol; they stain red after treatment with safranin |
| Gram positive | Bacteria that retain the crystal violet color after decolorizing by alcohol; they stain dark purple. |
| Counter stain | A second stain applied to a smear, provides contrast to the primary stain. |
| decolorizer | A solution used in the process of removing a stain. |
| Special stains | Are used to color and isolate specific parts of microorganisms, such as endospores and flagella, and to reveal the presence of capsules. |
| hypothesis | A proposed explanation for an observable phenomenon. |
| Theory | a hypothesis assumed for the sake of argument or investigation b : an unproved assumption |
| Scientific method | refers to a body of techniques for investigating phenomena, acquiring new knowledge, or correcting and integrating previous knowledge. |
| Independent variable | is that variable which is presumed to affect or determine a dependent variable. It can be changed as required, and its values do not represent a problem requiring explanation in an analysis, but are taken simply as given |
| dependent variable | is a variable dependent on another variable: the independent variable. In simple terms, the independent variable is said to cause an apparent change in, or simply affect, the dependent variable |
| Assumption | is a proposition that is taken for granted, as if it were true based upon presupposition without preponderance of the facts. |
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heatherleone81
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