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micro test 3
staph & strep test
| Question | Answer |
|---|---|
| 1.)what is the gram stain of staphylococcus? | Gram pos cocci clusters |
| 2.)what is the typical colony morphology of S.aureus? | yellow, opaque, beta hemolytic (halo,clear) |
| 3.)Describe the catalase test. | |
| A.)what reagent is used? | hydrogen peroxide |
| B.)what does a positive and negative result look like? | +bubbling -no bubbles |
| C.)which genus is catalase positive? | staphylococcus |
| D.)which genus is catalase negative? | Steptococcus |
| 4,)describe mannitol salt agar. | contains the sugar mannitol & high salt concentration 7.5-10% |
| A.)which organisms grow on the plate? | all staphylococci |
| B.)what does a positive reaction look like? | growth surrounded by yellow halo |
| C.)what does a negative reaction look like? | growth with no color change to media |
| D.)which organism is mannitol salt positive? | S.aureus |
| E.)which organism is mannitol salt negative? | S.epidermidis |
| 5.)what is an enterotoxin? | Exotoxin that causes GI Sx- N/V/D, abd pain. caused by S.aureus ie.food poisoning esp: cream soups, pastries, custards food handeld after cooking 2 hour rule |
| 6.)what are some infections caused by S.aureus? | A.) boils B.) pimples/acne C.)wound inf. incl post op D.)TSS-toxic shock syndrom. E.)meningitis F.)osteomyelities G.)Impetigo contagios. H.)HAI hospital acquired inf nosocomial I.)MRSA-methicillin resistan s.aureus skin to skin contact towles bandages |
| 7.)what is the characteristic lesion of S. aureus? | Abscess-pus forming ability, inability to spread. *infections of skin* |
| 8.)what does a typical colony of S.epidermidis look like? | white, opaque, non-hemolytic-clear halos in agar |
| 9.)where is S.epidermidis normal flora? | on the skin, throat, vagina |
| 10.)what is the gram stain of streptococcus? | gram pos cocci chains. 50 different species, more pathogens in this genus than any other |
| 11.)what does fastidious mean ? | needs more care to grow, difficult to grow |
| 12.)what does strep require in the atmosphere to grow? | Co2 |
| 13.)what are different methods to provide increased CO2? | A.)candle jar-unscented votive candle , put on top of stack.B.)CO2 generator envelope/sachet.C.)bag & tablet.D.)CO2 incubator |
| 14.)what is the typical colony morphology of streptocococcus? | gray, translucent |
| 15.)Define the following. | |
| A.)alpha hemolysis; | green halo surrounding colony. hemolysis slight or incomplete sub-accurte chronic infs. tooth abscess |
| B.)beta hemolysis | clear, colorless halo. No intact RBCs accute infs strep. |
| C.)gamma hemolysis: | no change in blood surrounding colony/none |
| 16.)which Lancefield group of the beta streptococcus is most pathogenic? | Group A-S.pyogenes causes strep throat quick step test to be pos. only one that causes. groups A-0 bases on antigen on cell wall. |
| 17.)which lancefield group causes infection in newborns? | group B |
| 18.)what does GBS stand for? | group B strep. norm flora in 25% of healthy woman in vagina,intestine and rectum. can cause sepsis in infants can get is as they pass thru the vagina during birth. |
| 19.)describe the bacitracin disk test. | |
| A.)what organism is it used on? | beta hemolytic strep |
| B.)what does a positive result look like? | =to a zone of growth inhibition. red around disk bacteria cant get in there. |
| C.)what does a negative result look like? | =to no zone of growth inhibition growth up to disk. |
| D.)which organism is positive? | Group A beta hemolytic strep |
| E.)which organism is negative? | not group A beta hemolytic strep |
| 20.)what is the characteristic lesion of beta hemolytic streptococci? | cellulitis=fluid given off by damaged connective tissue, fluid few cells, little fibrin |
| 18.)test woman at 36 weeks if pos will give IV antibiotics during L & D | |
| 21.)what are several infections caused by group A beta hemolytic streptococci? | A.)strep throat B.)scarlet fever (2-6 yr old) same as strep throat.C.)AGN-acute glomerulonephritis=infl of glomerulus of kidney.D.)RF-rheumatic fever E.)pneumonia, meningitis wound infections. C&D allergic result of strep inf. |
| 22.)what is an erthrogenic toxin? | exotoxin that causes a rash. |
| 23.)what is droplet infection? | fine spray given off by sneezing, coughing & talking |
| 24.)where is alpha hemolytic streptococci normal flora? | mouth, throat resp tract. |
| 25.)what is one infection alpha hemolytic streptococci can cause? | SBE-subacute bacterial endocarditis. Dentisity-can get in by teeth cleaning & can affect those with cardia problems. |
| 21.)if strep produces exotoxin * pt not immune=SF(can only get SF once) if strp doesnt produce or if pt is immune=strep throat |
Created by:
Buta610
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