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Lab Exam 2 Micro
| Question | Answer |
|---|---|
| Purpose of spread plating | to create isolated colonies by evenly spreading on plate, creating a bacterial lawn |
| Difference between streak plating and spread plating | Streak plating uses flamed loop to spread and decrease bacteria Spread plating uses flamed alcohol rod to spread and decrease bacteria |
| What is the zone of inhibition | The clear zone around antibiotic disk where bacterial growth is inhibited |
| How to determine if an organism is susceptible or resistant to an antibiotic | Measure zone diameter and then use Antibiotic Disc Info Sheet |
| How does the antibiotic get into the agar | Antibiotic diffuses into agar causing a concentration gradiant, by using an antibiotic disk dispenser to place disks before incubation |
| What is the purpose of the Synder Test | Measures susceptibility to dental carries (tooth decay) |
| How do you interpret Synder test results | With color change of media |
| Explain what the pH is with the Snyder test | The medium favors growth of oral bacteria pH 4.8 or higher it is GREEN & pH lower than 4.8 it turns YELLOW |
| Explain how the pH works with the Snyder test | When oral bacteria uses glucose as an energy source they produce acid as a by product, acid lowers the pH, thus changing the color in the test (same acid that erodes away tooth enamel) |
| What is a biofilm | 2 or more microorganisms that secrete an extracellular matrix and adheres to a surface |
| What is nosocomial infection | An infection that was acquired in a health care setting when receiving care |
| Why were Staphylococcus aureus and Staphylococcus epidermidis chosen for the clinical biofilm experiment | 1) causes biofilms on medical equipment causing nosocomial infections 2) both organisms are always present on the skin |
| What are MMWR's | The CDC collects weekly data on 26 notifiable diseases and their occurrence in the US and its territories |
| What does MMWR mean | Morbidity and Mortality Weekly Reports |
| Equation for calculating MMWR incident rate | Incident rate = # of new cases in a time period / size of at risk population at midpoint of time period x K |
| What is the incident rate | occurrence of new cases of a disease within a defined population and specific time |
| How to determine index case in epidemic | Ro - represents number of people a single person can transmit disease to |
| Saccharomyces cerevisiae | Classification: Ascomyota Disease: Vaginitis - used in production of bread/beer |
| Candida Albicans | Classification: Ascomyota Disease: Yeast infection |
| Rhizopus | Classification: Zygomycota Disease: Zygomycosis |
| Penicillium | Classification: Ascomyota Disease: Infections of lung, liver, and skin |
| Aspergillus | Classification: Asomyota Disease: Aspergillosis |
| Entamoeba Histolytica | Classification: Amoebozoa Disease: Amebic dysentery |
| Giardia Lamblia | Classification: Diplomonadida Disease: GI infection |
| Trichomonas Vaginalis | Classification: Parabasala Disease: Trichomoniasis |
| Trypanosoma | Classification: African Sleeping Sickness Disease: Euglenozoa |
| Plasmodium | Classification: Alveolates Disease: Malaria |
| Balantidium coli | Classification: Alveolates Disease: GI infection |
| Ascaris Lumbricoides | Classification: Nemotodes/ round worms Disease: Ascaraisis (contaminated food/water) |
| Necator Americanus | Classification: Nemotodes/ round worms Disease: Hook worm infection |
| Schistosoma Mansoni | Classification: Trematodes/ flukes Disease: "snail fever" Infection |
| Taenia Dipylidium(Caninum) | Classification: Cestodes Disease: Tapeworm infection |
Created by:
barberalora11
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