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Plant bio
C3 carbon fixation
| Question | Answer |
|---|---|
| How does carbon enter plants? | - as CO2 through the stomates |
| How does CO2 enter into photosyntheic tissue? | - diffusion of molecules down concentration gradients |
| Incorporation of 14C CO2 into photosynthetic products | - overtime, organic phosphates, malate, aspartate, alanine increase total % incorporation |
| Photosynthetic metabolite pool sizes are responsive to light | - 3-phosphoglycerate pool size increases in dark, but dips in light - Ribulose bisphosphate pool size dips in dark, but increases in light |
| Rubisco | - major enzyme that catalyzes the primary chemical reaction by which inorganic carbon enters the biosphere |
| CO2 entry into the Calvin cycle | In the Calvin cycle, carbon atoms from CO2 are fixed (incorporated into organic molecules) and used to build three-carbon sugars. This process is fueled by, and dependent on, ATP and NADPH from the light reaction, and takes place in the stroma |
| Metabolic fate of triphosphate | Carbon balance of chloroplast In Chloroplast: starch synthesis from triose phosphate reversal of glycolysis initially Triose phosphate is exchanged with phosphate In cytosol: sucrose synthesis from triose phosphate reversal of glycolysis initially |
| Gas exchange parameters | Stomatal conductance – water vapour loss, units CO2 balance - net assimilation CO2 internal - units |
| Net CO2 fixation | - balance between respiration & photosynthesis |