Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Histology

lab 1

QuestionAnswer
What is A in sections of a tube longitudinal
What is B in sections of a tube tangential
What is F in sections of a tube transvers/cross
Structures that were not originally present in living tissue formation Artefacts
reasoning for artefact Poor tissue prep
reasoning for artefact improper fixation
reasoning for artefact type of fixation (issue)
reasoning for artefact poor dehydration
What is A in longitudinal section of round object Midline
What is B in longitudinal section of round object Peripheral
What is C in longitudinal section of round object Tangential
What is D in Transverse section of round object Midline
What is E in Transverse section of round object Peripheral
What is F in Transverse section of round object Tangential
What is C in sections of a tube oblique
What is D, E in sections of a tube transvers/cross
reasoning for artefact improper reagents
reasoning for artefact contamination
reasoning for artefact surgical procedure
reasoning for artefact poor microtome sectioning
the shortness distances between two separated points in microscope field of view that can be distinguished as a distinct entities Power of resolutions
power of the eyepiece (10x or 12.5x) multiplied by the power of the objective (4x, 10x, 20x, 40x, 60x, 100x) magnification of the microscope
Universally used staining method for routine histo examination of tissue sections Hematoxylin and Eosin Staining
What type and color does Hematox. stain stains acidic molecules shades of blue
Name of structures that bind to Hematox. Basophilic
What color does Eosin stain Red, pink, orange
Names of structures that bind to Eosin Eosinophilic or acidophilic
what does Hematox. stain Colors nuclei of cell blue
What does Eosin stain colors cytoplasm and other structure red/pink
steps in prep for histo. slides Specimen collection, Fixing, processing, embedding, sectioning, staining, analysis
Specimen collection Post mortem (necropsy) or direct extraction (biopsy)
Fixing is based on... saving tissue as close to its living state as possible
Why fix tissues so it will not change its volume/shape during processing to prepare tissue and leave in condition that allows clear staining sections
what does fixing prevent autolysis and bacterial attack
types of fixing acetic acid, formaldehyde (formaline), glutaraldehyde, ethanol, freezing with liquid nitrogen or methanol
factors effecting fixation volume of tissue (recommended 1cmx1cmx1cm) ration of fixative to tissue ratio (10:1), temp, pH, penetration of fixative
processing Dehydration then clearing
dehydration for processing to remove fixative and water from the tissue and replace them w dehydrating fluid; dehydrate in graded ethanol series from lower to higher % of alc (70%, 80, 90, 96, 99.8%)
Clearing for processing replacing dehydrating fluid w fluid that is miscible w both the dehydrating fluid and the embedding medium (XYLENE)
process by which specimen is surround by an embedding medium like paraffin wax Embedding
paraffin solidifies and the specimen can be cut during sectioning purpose of paraffin wax
to cut specimen into very thin sections for microscopic examination (3-5 mu m), very thin (1500 nanometers) sectioning
Dewaxing, hydration, staining, dehydration Staining
removing paraffin by Xylene Dewaxing
in graded ethanol series, from high to low, (99.8%, 96, 90, 80, 70%) hydration for staining
Mounting slides Analysis
mounting medium places ontop of slide and covered by very thin coverslip how to mount slides
Created by: j_kasprzak
Popular Science sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards