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Effect of Temp

Effect of Temperature on rate of enzyme reaction - Leaving Cert Biology

QuestionAnswer
What is the first step in the Temperature Enzyme experiment? Place some pH buffer 9 solution in a graduated cylinder
What is the reason for using a pH buffer? To ensure that the solution stays at the constant pH of pH 9.
What is the 2nd step of the Temp experiment? Add one drop of washing up liquid to the graduated cylinder.
The 3rd step of the the Temp experiment? Blend some celery stalks in and blender, then filter into a beaker with filter paper
4th step of the Temp experiment? Add filtered solution to the graduated cylinder .
Fifth step of the Temp experiment? Add Hydrogen Peroxide (H2O2) to a smaller graduated cylinder.
What is the 6th step of the Temp Experiment? Place both graduated cylinders in an ice bath until they reach 0 degrees Celsius.
Seventh step of the Temp experiment? Remove both graduated cylinders from the ice bath and add the H2O2 to the graduated cylinder containing the celery solution.
The 8th step of the Temp experiment? Note and record the volume at the top of the foam after two minutes.
The ninth step of the Temp experiment? Calculate the volume of foam formed/produced.
What should be done after the calculation of the volume of foam formed? Steps 1- 9 should be repeated at 10,20,30,40,50 and 60 degrees Celsius and the results from all the experiments should then be used to create a graph.
Created by: AW117
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