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Enzymes Bio
Enzymes Biology Leaving Cert
| Question | Answer |
|---|---|
| What is Metabolism? | The total of all chemical reactions within and organism. Such as growth, homeostasis, digestion etc. |
| How does the body get energy? | All energy comes from producers in the food chain (plants). They trap solar energy during photosynthesis, creating carbohydrates and other biomolecules. This energy will be released by carbohydrates and lipids that we consume. This is Cellular energy. |
| What is Cellular energy used for? | The energy released by some of the consumed carbohydrates and lipids is used in aerobic and anaerobic respiration. The rest of the energy is released into the atmosphere as heat. |
| What are Enzymes? | Enzymes are biological catalysts made of amino acids. They speed up chemical reactions in organisms. If they denature due to change in pH and/or temperature, their shape will change irreversibly and stop working. |
| What are the roles of Enzymes? | - Catabolic Enzymes break down molecules into smaller molecules ie. Amylase breaks down starch into Maltose - Anabolic enzymes build up molecules from smaller molecules ie. DNA Lipase joins DNA together. |
| Factors that effect Enzyme activity. | - pH - most human enzymes work at an optimum of pH 5 and most plant enzymes work at an optimum temperature of pH 9 - Temperature - Human enzymes work at an optimum of 36-37 degrees Celsius, whereas plant enzymes optimum temperature is 20-30 degrees C |
| Uses of Immobilised enzymes. | - Used in Bioprocessing for making antibodies, vitamins and amino acids etc - yeast and bacteria in food and drink. |
| What is one of the benefits of using Immobilised enzymes in bioprocessing? | The Immobilised enzymes can be reused so the process becomes less expensive. |
| What is an active site? | An active site is a depression in the surface of an enzyme and is larger than the substrate. |
| What does optimum mean? | Optimum means the correct/perfect conditions for something to work to the best of their ability. For example enzymes such as protease in the body working best at 36 degrees and pH of 2. |
| What is Mechanism of Enzyme Action? | This is where the Substrate combines with the active site which changes slightly to fit substrate. The substrate is then broken down into products. The active site will then return to its original shape. |
| What is Denaturation? | The irreversible change in shape of an enzyme that had been exposed to the incorrect conditions like wrong pH and temperatures over 40 degrees Celsius. |
| Enzyme Carriers - Animals | - ADP - Adosine DiPhosphate . Bond: Adosine-Ribose -Phosphate-phosphate - ATP - Adosine ThioPhosphate . Bond: Adosine-Ribose-Phosphate-Phosphate-Phosphate. |
| Enzyme Carrier - Plants | - NADP+ - Nicotinamide Adenine Dinucleotide Phosphate. -NADPH - Nicotinamide Adenine Dinucleotide Phosphate Hydrogen. - NAD+ - Nicotinamide Adenine Dinucleotide - NADH+ - Nicotinamide Adenine Dinculeotide. |