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Microbiology

Bacteria Lab results/ enzymes

QuestionAnswer
Aerotolerance ability or inability to grow in O2
Obligate Aerobes only grow in o2
microaerophiles can live in reduced O2
Obligate anaerobes Can NOT live in any O2 Fermentors
Facutative Anrobes Aerotolerant anaerobes don't require O2, but not adversely affected by it
Fluid Thioglycollate Medium liquid pancreatic digest marker for oxidation grows all aerotolerances
Anerobic Jar chemical gas generator packet removes all O2
Respiration glucose converted to atp by 1) glycolysis 2)kreb 3) oxidative phosphorylation ETC
Glycolysis splits the 6 carbon glucose into two three carbon pyruvate molecules with the production of ATP and reduced coenzymes
Electron transport chain oxidation-reduction reactions, receives electrons from the reduced coenzymes produced during glycolysis. Final electron acceptor; when O2 is final electron acceptor it is aerobic; when an inorganic molecule other than oxygen the respiration is anaerobic
Fermentation glucose acts as an electron donor and one or more of its organic products act as the final electron acceptor. Reduced carbon compounds (in forms of acids/organic solvents and CO2) are typical end products
Oxidation-Fermentation Test differentiate bacteria based on the ability to oxidize or ferment specific sugars; allows preemptive separation of fermentative enterobacteriaceae from the oxidative pseudomonas and bordetella, and the nonreactive alcaligenes and moraxella
Phenol Red Broth broth is used to differentiate members of enterobacteriaceae and to distinguish them from other gram-negative rods
Methyl red and Voges-Proskauer Tests differentiate enterobacteriaceae from other gram-negative rods MR: detects B capablre of mixed acid fermentations VP: B able to ferment glucose to acetoin
Catalase Test indentify organisims that produce the enzyme catalase; differentiate members of catalase-positive micrococcaceae and catalase negative streptococcaceae
Oxidase Test ETC of many aerobes, facukltative aerobes, microaerophiles have respiratory enzyme cytochrome c oxidase to transfer e- to O2
nitrate reduction test - may produce N2 gas (durham tubes) Anaerobic respiration = e- to inorganic molecule like Nitrite NO3 (nitrate reduction). ENZYME: nitrite reductase, reduces nitrate to nitrite NO2. Some B use DENIRIFICATION to enzymatically convert nitrate to N2 (gas)
Citrate test - checking for citrate utilizers Citrate (as aceytl co enzyme A) comes from oxidation of pyruvate or fatty acids, & prepare to enter Krebs cycle. Process can be reversed. If B possess Citrate-Permease they can produce Pyruvate from Citrate.
SIM Medium (combination differential media) multiple biochemical determinations from a single inoculation B reduce sulfer by ENZYMES: Sulfur: Cysteine desulfurase catalyzes AA cystine to pyruvate Indole: B producing enzyme Tryptophanse catalyse tryptophan to Indole Thiosulfate reductase catalyses reduction of sulfur (Sulfate) in ETC
Coagulase Tests Coagulase enzyme contributes to the resistance of pathogens to immune response and antimicrobials in Staphylococcus aureus Bound & Free. Rabbit Plasma Coagulation of plasma indicates POS
decarboxylation test Decarboxylase(Removes Carboxyl group COOH) (If organism is decarboxylase +, it produces alkaline end product which turn medium to basic)
phenylalanine deaminase test Phenylalanine deaminase [removes the amine group (NH2) from phenylalanine and produces NH3 and phenylpyruvic acid]
EC - escherichia-coli enterobacteria Oxidative (carbohydrates) Nitrate reduction to nitrite Citrate utilized for carbon Fermentor
SA- staphylococcus aureus faculitative ENZYMES: amalase
PA- pseudomonas aeruginosa Fermenter(carbohydrates) Gram negative aerobic
EA- enterobacter aerogenes Enzymes: Citrate permease Decarboxylase enterobacteriaceae
Created by: onesmartgirl
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