click below
click below
Normal Size Small Size show me how
Transcrip eukary
Chapter 17- transcriptional regulation in eukaryoties
Question | Answer |
---|---|
how are cis-acting refulatory elemtns, activators, and chromatin modifiers all cordinately involved in regulating transcription initiation ? | |
provide the definition of chromatin remodaling and give 2 examples | - one method of nucleosome modifiction in which remodeling complexes reposition/reconfigure nucleosomes using ATP as an energy source - replacment of histone H2A with histone variant H2A.Z and removal of nucleosomes bound to GAL promoters by chromatin re |
. Describe how DNase I resistance or sensitivity might indicate transcriptional activity. | |
Present an overview of the manner in which chromatin can be remodeled. Describe the manner in which these remodeling pro- cesses influence transcription. | |
Explain how the addition of acetyl groups to histones leads to a weaker association of DNA in nucleosomes. | |
How are the effects of enhancers restricted so that they do not exert inappropriate transcriptional activation of non-target genes? | |
Compare the control of gene regulation in eukaryotes and bacteria at the level of initiation of transcription. | |
Research indicates that promoters may fall into one of two classes: focused or dispersed. How do these classes differ, and which genes tend to be associated with each? | |
Many transcriptional activators are proteins with a DNA-binding domain (DBD) and an activation domain (AD). Explain how each domain contributes to transcriptional initiation. Would you expect repressors to also have each of these domains? | |
Using a diagram, suggest a way in which supercoiling may positively influence enhancer activity over long distances. |