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micro lab 3
| Question | Answer |
|---|---|
| does refrigeration inhibit growth (lab 19) | yes but it doesn't kill bacteria |
| what does antiseptic do (lab 20) | removes most transient flora on the skin biota |
| what do wipes and alcohol kill (lab 20) | the pathogens |
| the more we wash our hands the more bacteria colonies appear. what does that mean? (lab 20) | it removes dead skin tissues so more microbiota shows up |
| why does antiseptic work better when it's held on the fingers (lab 20) | it needs time to work |
| what is the definition of minimal inhibitory concentration (lab 21) | the lowest concentration of the drug that prevents growth |
| why did we swab the bacteria in every direction (lab 21) | so the bacteria covers the plate completely |
| if you were given several diameters larger to smaller which is the most effective and least effective (lab 21) | more space more effective least space least effective |
| water moves across a semipermeable membrane but ___&___ doesn't (lab 22) | salt & sugar |
| what helps the cell in hypertonic conditions (lab 22) | the solute concentration surrounding a bacterium is very low in relation to the solute concentration within the bacterium |
| what makes the cell shrink and what makes it swell (lab 22) | water moving into the bacterium will make it swell the rigid cell wall prevents it from lysing(bursting) |
| definition of isotonic solution (lab 22) | the solute concentration outside the cell is equal to the solute concentration inside the cell |
| what does pH indicate (lab 23) | H+ ions in a solution |
| between acidic and bases where do we get a high concentration of H ion (lab 23) | acidic |
| does boiling kill endospores (lab 24) | no it kills the vegetive cells |
| what are the standard conditions of an autoclave (lab 24) | 15/lb in^2 and 100-121 C for 15 minutes |
| what kind of medium is LSB (lab 25) | selective and differential |
| what is the positive result when we use LSB for water testing (lab 25) | gas production |
| characteristics of coliform (lab 25) | gram negative, non-spore-forming rods, ferment lactose to produce acid and CO2 gas |
| what does the most probable number indicate (lab 25) |