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Biology Lab Exam
Questions from Pre and Post Lab Quizzes
| Question | Answer |
|---|---|
| What pH is optimum for human enzymes generally? | 7 |
| What pH is optimum for stomach enzymes? | 2 |
| What temperature is optimum for human enzymes? | 37 C |
| What is the temperature tolerance for microbes that live in extreme temperatures? | 100 C |
| Beano is an over the counter remedy that helps people take the complex carbohydrates in beans and change them to simple sugars like glucose? S+E -----> P+E What is P? | simple sugars, like glucose (product) |
| Beano is an over the counter remedy that helps people take the complex carbohydrates in beans and change them to simple sugars like glucose? S+E -----> P+E What is E? | Beano (enzyme) |
| Beano is an over the counter remedy that helps people take the complex carbohydrates in beans and change them to simple sugars like glucose? S+E -----> P+E What is S? | Complex carbohydrates (substrate) |
| "Scientists never present raw data, data directly entered in a notebook as it is collected, in presentations or journal articles. Instead, they create figures and tables that summarize the data. If done correctly, the reader can easily and clearly..." | understand the detailed results and see complex relationships, patterns, and trends |
| pH of 12 or higher | basic |
| pure water pH | 7 (neutral) |
| pH 3 or less | acidic |
| what is the most important part of a hypothesis in science? | it is testable |
| What does the coloration with the dye accomplish according to the lab? | Dyes enhance the features of the cell |
| Look at picture from Pre Lab 4 what do you expect if the person gets an infection? | more immune cells |
| Look at picture from Pre Lab 4 As we age, we can lose the ability to fight off infections. What do you expect from a much older person if their immune cells are failing? | less immune cells |
| Look at picture from Pre Lab 4 what is the ratio of red blood cells to immune cells? | lots more red blood cells than immune cells |
| Look at picture from Pre Lab 4 Athletes train at high elevation to increase the amount of oxygen. What should happen to their blood smear? | more red blood cells |
| what is a characteristic of plant cells? | rigid cell walls from cellulose, often looking like blocks under a microscope |
| How much magnification you get depends on the eye-piece/ocular lens power times the objective lens power. If the eye piece is 7.5 power and the ocular is 10 power, how much magnification is there? | 75 power |
| Size of a red blood cell in a human body | 8 μm (micrometers) |
| Put these in order, from largest at top, to smallest at the bottom using your knowledge of cell size. Nucleus of a eukaryotic cell Chloroplast of plant, Eukaryotic cell Cheek cell-human and eukaryotic One E.coli bacteria- prokaryotic | Cheek cell-human and eukaryotic Nucleus of eukaryotic cell Chloroplast of plant, eukaryotic cell One E.coli bacteria-prokaryotic |
| why is the free market failing the health care market for antibiotics? | There is no global market for antibiotics Returns on the investments to make new antibiotics are low A really good antibiotic, shouldn't be used very much Evolution of the bacteria is faster than antibiotics |
| On our graphs for class...Should there be graph gridlines? | NO |
| On our graphs for class...Where should the legend be? | on the right and inside the graph box |
| Drug companies will discontinue the trial of about 1/3 of the new antibiotics. If there are 12 new antibiotics starting clinical trials, how many would be discontinued by this estimate? | 4 |
| According to the article that we read for Post Lab 5 about antibiotic resistance... | Acquired resistance that leads to failure of infection treatments is currently a subject of concern. Untreated sewage is a factor Triclosan, used in a wide range of consumer products, has been banned or restricted in various markets. |
| what are the ways resistance can be acquired? Choose all that apply Mutation in bacterial DNA Horizontal gene transfer Sharing DNA between two different bacteria Engulfing to consume another bacterium that has the resistance | Mutation in bacterial DNA Horizontal gene transfer Sharing DNA between two different bacteria |
| How do antibiotics get in the environment? | Spreading manures, poor or improper treatment of wastewater, agricultural run-off, release of microorganisms to inhibit their competitors |
| How are we combating the rise in antibiotic resistance? | Reducing the use of antibiotics in agriculture, unless animal is sick Banning Triclosan, used in a wide range of consumer products like antibacterial soap Improving wastewater treatment to remove antibiotics, like UV and heat |
| The 2014 O'Neill report commissioned by the UK government estimated that antimicrobial resistant infections may become the leading cause of death globally by 2050. True or False? | True |
| What is coliscan media selective for? | gram negative bacteria |
| If E. coli grows on the coliscan plate what color does it turn? | blue-purple |
| Galactosidase enzyme activity in coliscan media gives a | pink color to colonies |
| Glucronidase enzyme activity in coliscan media gives a | greenish color to colonies |
| The bacteria E. coli can be described in many ways. Below are statements, if they apply to E. coli, you should select those choice(s). Grows on EMB - eosin methylene blue Not a coliform bacteria Fecal coliform Gram positive Gram negative | Grows on EMB- eosin methylene blue Fecal coliform Gram negative |
| Since we added tetracycline to the coliscan media, the bacteria we found on the plates should have been tetracycline resistant.. True or False? | True |
| When bacteria is _________ to an antibiotic, this means the bacteria can grow even if the antibiotic is present in the environment. If the bacteria is ________ to the antibiotic, this means the antibiotic kills the bacteria so it does not grow. | resistant; sensitive |
| We are trying to determine if E. coli from the North River have antibiotic resistance. What is the order of the lab steps? | 1. Sample water 2. Filter water to grid 3. Use coliscan media to grow bacteria 4. Select an E. coli colony 5. Use EMB media to grow bacteria 6. Grow bacteria in a broth or liquid culture 7. Test bacteria on TSA plates with tetracycline strips |
| What color is E. coli on an EMB plate? What causes this? | Lime green. The fermentation of lactose changes the pH in the dyes on the plate |
| The coliscan media selects for ______ and the deep blue or purple color colony or colonies were ______. | Gram (-); E. coli |
| What are the 2 types of dyes on the EMB plate and what do they do? | Eosin Y and Methylene blue. They do not let Gram (+) bacteria grow |
| What is the CRAP test? | Currency, Reliability, Authority, Purpose |
| In the lab introduction, your faculty point out it is very important to understand when resistance develops in bacteria exposed to antibiotics. When it that? | Before the bacteria are exposed |
| The lab 9 sheet says the clear zone on the plate cannot tell the difference between __ meaning killing the bacteria and __ meaning the bacteria just cannot grow. | bacteriostatic; bacteriocidal |
| What happens if there is no zone of inhibition? | The antibiotic was ineffective on the growth of the bacteria |
Created by:
abbiemichael03
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