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Biology
Physical and chemical methods
| Term | Definition |
|---|---|
| Germicidal | Methods KILL microorganisms "Germs" |
| Bactericidal | Kill Bacteria |
| Bacteriostatic | Only inhibit bacterial growth |
| Sporicidal | Kill bacterial endospores |
| Fungicidal | Kill fungi |
| Fungistatic | only inhibit fungal growth |
| Virucidal | inactivates viruses |
| Sterilization/Sterilant/Sterile | The process of the destruction and complete removal of all viable microorganisms from inanimate surfaces |
| Disinfection/Disinfectant/Disinfected | |
| Antisepsis/Antiseptic | |
| Sanitization/Sanitizer | |
| Decontamination | |
| Preservation | |
| Mode of action | |
| Heat | |
| Dry Heat | |
| Moist heat | |
| Pasteurization | |
| Boiling water | |
| Steam heat under pressure | |
| Autoclave | |
| Low temperatures | |
| Refrigeration | |
| Radiation | |
| UV irradiation | |
| Gamma irradiation | |
| Filtration | |
| Dehydration/Desiccation | |
| Halogens | |
| Chlorine | |
| Chlorites | |
| Aldehydes | |
| Formaldehyde | |
| Glutaraldehyde | |
| Hydrogen peroxide | |
| Ethanol/ethyl alcohol | |
| Isopropanol/isopropyl alcohol | |
| Chlorhexidine | |
| Phenolics | |
| Triclosan | |
| Quaternary ammonium cmpds | |
| Amphipathic | |
| Vinegar/acetic acid | |
| Ammonia | |
| Heavy metals | |
| Osmolarity | |
| Salinity | |
| CHEMICAL METHODS | |
| Halogens what's their mode of action? | halogens: Chlorine/ chlorites ex. Bleach, and Iodine Action: Damage proteins Bactericidal (BC) DOES:Disinfect |
| Aldehydes what's their mode of action? | Aldehydes: Formaldehyde and Glutaraldehyde Action: denature proteins Bactericidal (BC) DOES: Sterilize |
| Hydrogen peroxide what's their mode of action? | Hydrogen peroxide: 3% and 10% Action: Damage proteins Bactericidal (BC) DOES: Disinfect or Sterilize |
| Alcohols what's their mode of action? | Alcohols: Ethanol and Isopropanol Action: Damage proteins Bactericidal (BC) DOES: Disinfect |
| Chlorohexidine what's their mode of action? | Action: Damage cell membranes Bactericidal (BC) DOES: Disinfect |
| Phenolics what's their mode of action? | Action: Damage protein and membranes Bactericidal (BC) DOES: Disinfect |
| Quaternary ammonium what's their mode of action? | Action: Damage membranes Bactericidal (BC) DOES: Disinfect |
| Acids what's their mode of action? | Action: Damage or denature proteins Bactericidal (BC) DOES: Disinfect |
| Heavy metals what's their mode of action? | Action: Denature proteins Bactericidal (BC) DOES: Preserve |
| Osmolarity what's their mode of action? | Action: Dehydrate cells is Bacteriostatic (BS) DOES: Preserve |
| PHYSICAL/ MECHANICAL METHODS | |
| Dry Heat | Action: Denature protein - is Bactericidal DOES: Sterilize |
| Moist Heat | --- |
| Pasteurization | Action: Denature protein- is Bactericidal DOES: Disinfect |
| Boiling Water | Action: Denature protein- is Bactericidal DOES: Disinfect |
| Steam heat under pressure (Autoclave) | Action: Denature protein- is Bactericidal DOES: Sterilize |
| Cold Temps (Refrigeration/ freezing) | DOES: To Preserve |
| Radiation | Action: Damage DNA- is Bactericidal |
| Non- Ionizing radiation (UV) | Action: Damage DNA-is Bactericidal DOES: Disinfect |
| Ionizing Radiation (Gamma) | Action: Damage DNA-is Bactericidal DOES: Sterilize |
| Filtration | Decontamination- DOES: Sterilize and disinfect |
| Desiccation | Action: Dehydrate cell- is Bacteriostatic DOES: Preserve |