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Biology

Physical and chemical methods

TermDefinition
Germicidal Methods KILL microorganisms "Germs"
Bactericidal Kill Bacteria
Bacteriostatic Only inhibit bacterial growth
Sporicidal Kill bacterial endospores
Fungicidal Kill fungi
Fungistatic only inhibit fungal growth
Virucidal inactivates viruses
Sterilization/Sterilant/Sterile The process of the destruction and complete removal of all viable microorganisms from inanimate surfaces
Disinfection/Disinfectant/Disinfected
Antisepsis/Antiseptic
Sanitization/Sanitizer
Decontamination
Preservation
Mode of action
Heat
Dry Heat
Moist heat
Pasteurization
Boiling water
Steam heat under pressure
Autoclave
Low temperatures
Refrigeration
Radiation
UV irradiation
Gamma irradiation
Filtration
Dehydration/Desiccation
Halogens
Chlorine
Chlorites
Aldehydes
Formaldehyde
Glutaraldehyde
Hydrogen peroxide
Ethanol/ethyl alcohol
Isopropanol/isopropyl alcohol
Chlorhexidine
Phenolics
Triclosan
Quaternary ammonium cmpds
Amphipathic
Vinegar/acetic acid
Ammonia
Heavy metals
Osmolarity
Salinity
CHEMICAL METHODS
Halogens what's their mode of action? halogens: Chlorine/ chlorites ex. Bleach, and Iodine Action: Damage proteins Bactericidal (BC) DOES:Disinfect
Aldehydes what's their mode of action? Aldehydes: Formaldehyde and Glutaraldehyde Action: denature proteins Bactericidal (BC) DOES: Sterilize
Hydrogen peroxide what's their mode of action? Hydrogen peroxide: 3% and 10% Action: Damage proteins Bactericidal (BC) DOES: Disinfect or Sterilize
Alcohols what's their mode of action? Alcohols: Ethanol and Isopropanol Action: Damage proteins Bactericidal (BC) DOES: Disinfect
Chlorohexidine what's their mode of action? Action: Damage cell membranes Bactericidal (BC) DOES: Disinfect
Phenolics what's their mode of action? Action: Damage protein and membranes Bactericidal (BC) DOES: Disinfect
Quaternary ammonium what's their mode of action? Action: Damage membranes Bactericidal (BC) DOES: Disinfect
Acids what's their mode of action? Action: Damage or denature proteins Bactericidal (BC) DOES: Disinfect
Heavy metals what's their mode of action? Action: Denature proteins Bactericidal (BC) DOES: Preserve
Osmolarity what's their mode of action? Action: Dehydrate cells is Bacteriostatic (BS) DOES: Preserve
PHYSICAL/ MECHANICAL METHODS
Dry Heat Action: Denature protein - is Bactericidal DOES: Sterilize
Moist Heat ---
Pasteurization Action: Denature protein- is Bactericidal DOES: Disinfect
Boiling Water Action: Denature protein- is Bactericidal DOES: Disinfect
Steam heat under pressure (Autoclave) Action: Denature protein- is Bactericidal DOES: Sterilize
Cold Temps (Refrigeration/ freezing) DOES: To Preserve
Radiation Action: Damage DNA- is Bactericidal
Non- Ionizing radiation (UV) Action: Damage DNA-is Bactericidal DOES: Disinfect
Ionizing Radiation (Gamma) Action: Damage DNA-is Bactericidal DOES: Sterilize
Filtration Decontamination- DOES: Sterilize and disinfect
Desiccation Action: Dehydrate cell- is Bacteriostatic DOES: Preserve
Created by: mebathrick24
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