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micro test 3

summary Q

QuestionAnswer
better growth on SBA than MAC Oxidase +, TSI (K/K) Suspect a NF
found where moisture accumulates, all have pyoverdin Pseudomonads
Cystic fibrosos, Jacuzzi/hot tub syndrome, swimmers ear P.Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days aeruginosa
Virulence factors: attachment, invasion, toxins P. aeruginosa
lab ID: lg colonies, pyocyanin, “grape-like odor”, Oxi +, TSA +(green slant), growth at 42 C, Glucose +, Citrate + P. aeruginosa
needs 2x grug therapy P. aeruginosa
differentiated by gelatin hydrolysis P. flurescens +, P. putida (nasty odor) =
soil denitifier P. stutzeri
used to belong to Pseudomonas Burkholderia
onion bulb rot (plant pathogen), found in IV fluids, detergents, disinfectants Burkholderia cepacia
Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days Burkholderia cepacia
motile+, lysine/ornathine+, glucose/maltose/lactose/mannitol+ Burkholderia cepacia
“glanders”(respiratory disease in animals), NON-MOTILE B. mallei
meliodosis (aggressive granelomatous pulmonary disease) B. psuedomallei
3rd most NF in nosocomial infections, aerobic, motile by flagella, implants self in medical devices Stenotrophomonas maltophilia
-“ammonia odor” , Oxi/Cat + , Esculin +, Maltose+++++(strong) Stenotrophomonas maltophilia
MAC growth (purple), Oxi=, Cat + , Nonmotile nonmotile Acinetobacter
seen in nosocomial infectons: ICU Acinetobacter
contamination, nonsaccharolytic A.iwoffii
saccharolytic (glucose +) A. Baumannii
live in seawater thus Halophilic except V. chorela Vibrio
Oxi+, MAC growth, comma-shaped/curved rods Vibrio
cause CHOLERA a water borne illness (severe gastroenteritis) found in plankton, fresh, blackish, and salt water V. cholera
cholera toxin/Choleragen; “rice water stool” V. cholera
non hemolytic, VP= : classic V. cholera 01 beta-hemolytic, VP+ : el tor V. cholera 01 yellow on TCBS, sucrose+ V. cholera
growth 1-2% NCl, gastroenteritis from seafood(shell fish/ousters), TCBS green(sucrose=) V. parahemolyticus
wound infections then septicemia from raw shellfish/oysters, lactose+: V. vulificus
Oxi+, glucose+, found in flesh water, warm/cold blooded animal disease, motile by 1 flagella, grow 0-42 C Aeromonas
MAC/SBA(beta) growth, Oxi/Ind+ aerolysin (cytotoxic enterotoxin) A. hydrophilia
found in fresh water, growth SBA/MAC, motile Plasiomonas
cause gastritis from cold blooded animals P. shigelloides
-“darting motility”,curved/S shaped, “seagull shape” most common cause of diarrhea Capylobacter jejuni
microphilic, Cat/Oxi+, NLF, growth 42 C, Hippurate hydrolysis +, Campy-blood agar(sodium bisulfide) Capylobacter jejuni
curved, microaerophilic, high humidity Cat/Oxi/Urease+; EIA, urease test on biopsy, direct stain, stool antigen test Helibacter pylori
ulcers, gastric cancers Helibacter pylori
motile, Oxi + , growth MAC NLF, Nitrite to gas Alcaligenes faecalis
feather edge, “fruity” odor, groth in 6.5% NaCL Alcaligenes faecalis
-motile respiratory pathogens Bordetella bronchiseptica
non-motile respiratory pathogens Bordetella pertussis/parapertussis
pertussin toxin, tracheal cytotoxin, hemoaggutination Bordetella
catarrhal/paradxysmal/convalescent stage pertussis toxin
-temporal vaccine available -DFA/culture from nasal specimen Bordetella
Bordet-Genou/Regan-Lowe Bordetella
milder pertussis, nontoxigenic strain, Cat+Oxi=Urea+(24 hrs) B. parapertussis
Kennel cough in dogs, growth SBA/MAC, Cat/Oxi+Urea+(4 hrs): B. bronchiseptica
Cat/Oxi +, 20-43 C temp. range L. pneumophilia
-Cysteine/iron, BCYE -found in water ex. Lakes/air conditioners, lives/multiply in protozoa/bronchial macrophages Legionella
-get from breathing in: Legionnaires disease(pneumonia)/ Pontiac fever -urine test detects antigen, DFA, serological Legionella
zoonotic disease Brucella
(most virulent) from sheep/goat B. melitensis
Cat/Oxi +, no carb., Brucella agar (5% horse/rabbit serum), “fine grains of sand” on gram stain Brucella
-survives RES system -Brucellosis, Undulant, Mediterranean, Malta fever Brucella
-get from dairy products, contact from animal -serology is the gold standard Brucella
-growth on SBA/CHOC, no MAC -Oxi/Cat/Ind +; Urea =;“sick TSI” Pasteurella multocida
-“musty” “mushroom” odor -get from animal contact esp. cat/dog bite (Cellulitis), respiratory tract/eye infect Pasteurella
-leading cause of endocarditic within genus -Cat/Oxi =, Nitrate +, glucose/lactose fermented Hemophilus aphrophilus
-dental infections; Cat + Oxi = -poor growth on SBA/CHOC, adhere to agar, “pointed star” Actinobacillus actinomycetemconitans
-associated exclusively with endocarditis -rosettes on gram stain -Oxi/Ind + ; cat/Nit = Cardiobacterium hominis
-fist fight/bite “clenched fist wound” -pits agar, “bleach odor” -Oxi/Nit + ; non motile ; non saccharolytic Eikenella corrodens
--plumb rods in chains; Oxi + Cat = ; ferment glucose -flora of pharynx in young children; Remel Rapid NH Kingella spp. denitrificans/kingae
better growth on SBA than MAC Oxidase +, TSI (K/K) Suspect a NF
found where moisture accumulates, all have pyoverdin Pseudomonads
Cystic fibrosos, Jacuzzi/hot tub syndrome, swimmers ear P.Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days aeruginosa
Virulence factors: attachment, invasion, toxins P. aeruginosa
lab ID: lg colonies, pyocyanin, “grape-like odor”, Oxi +, TSA +(green slant), growth at 42 C, Glucose +, Citrate + P. aeruginosa
needs 2x grug therapy P. aeruginosa
differentiated by gelatin hydrolysis P. flurescens +, P. putida (nasty odor) =
soil denitifier P. stutzeri
used to belong to Pseudomonas Burkholderia
onion bulb rot (plant pathogen), found in IV fluids, detergents, disinfectants Burkholderia cepacia
Growth on SBA, MAC, CHOC(green/yellow colonies), 47 days Burkholderia cepacia
motile+, lysine/ornathine+, glucose/maltose/lactose/mannitol+ Burkholderia cepacia
“glanders”(respiratory disease in animals), NON-MOTILE B. mallei
meliodosis (aggressive granelomatous pulmonary disease) B. psuedomallei
3rd most NF in nosocomial infections, aerobic, motile by flagella, implants self in medical devices Stenotrophomonas maltophilia
-“ammonia odor” , Oxi/Cat + , Esculin +, Maltose+++++(strong) Stenotrophomonas maltophilia
MAC growth (purple), Oxi=, Cat + , Nonmotile nonmotile Acinetobacter
seen in nosocomial infectons: ICU Acinetobacter
contamination, nonsaccharolytic A.iwoffii
saccharolytic (glucose +) A. Baumannii
live in seawater thus Halophilic except V. chorela Vibrio
Oxi+, MAC growth, comma-shaped/curved rods Vibrio
cause CHOLERA a water borne illness (severe gastroenteritis) found in plankton, fresh, blackish, and salt water V. cholera
cholera toxin/Choleragen; “rice water stool” V. cholera
non hemolytic, VP= : classic V. cholera 01 beta-hemolytic, VP+ : el tor V. cholera 01 yellow on TCBS, sucrose+ V. cholera
growth 1-2% NCl, gastroenteritis from seafood(shell fish/ousters), TCBS green(sucrose=) V. parahemolyticus
wound infections then septicemia from raw shellfish/oysters, lactose+: V. vulificus
Oxi+, glucose+, found in flesh water, warm/cold blooded animal disease, motile by 1 flagella, grow 0-42 C Aeromonas
MAC/SBA(beta) growth, Oxi/Ind+ aerolysin (cytotoxic enterotoxin) A. hydrophilia
found in fresh water, growth SBA/MAC, motile Plasiomonas
cause gastritis from cold blooded animals P. shigelloides
-“darting motility”,curved/S shaped, “seagull shape” most common cause of diarrhea Capylobacter jejuni
microphilic, Cat/Oxi+, NLF, growth 42 C, Hippurate hydrolysis +, Campy-blood agar(sodium bisulfide) Capylobacter jejuni
curved, microaerophilic, high humidity Cat/Oxi/Urease+; EIA, urease test on biopsy, direct stain, stool antigen test Helibacter pylori
ulcers, gastric cancers Helibacter pylori
motile, Oxi + , growth MAC NLF, Nitrite to gas Alcaligenes faecalis
feather edge, “fruity” odor, groth in 6.5% NaCL Alcaligenes faecalis
-motile respiratory pathogens Bordetella bronchiseptica
non-motile respiratory pathogens Bordetella pertussis/parapertussis
pertussin toxin, tracheal cytotoxin, hemoaggutination Bordetella
catarrhal/paradxysmal/convalescent stage pertussis toxin
-temporal vaccine available -DFA/culture from nasal specimen Bordetella
Bordet-Genou/Regan-Lowe Bordetella
milder pertussis, nontoxigenic strain, Cat+Oxi=Urea+(24 hrs) B. parapertussis
Kennel cough in dogs, growth SBA/MAC, Cat/Oxi+Urea+(4 hrs): B. bronchiseptica
Cat/Oxi +, 20-43 C temp. range L. pneumophilia
-Cysteine/iron, BCYE -found in water ex. Lakes/air conditioners, lives/multiply in protozoa/bronchial macrophages Legionella
-get from breathing in: Legionnaires disease(pneumonia)/ Pontiac fever -urine test detects antigen, DFA, serological Legionella
zoonotic disease Brucella
(most virulent) from sheep/goat B. melitensis
Cat/Oxi +, no carb., Brucella agar (5% horse/rabbit serum), “fine grains of sand” on gram stain Brucella
-survives RES system -Brucellosis, Undulant, Mediterranean, Malta fever Brucella
-get from dairy products, contact from animal -serology is the gold standard Brucella
-growth on SBA/CHOC, no MAC -Oxi/Cat/Ind +; Urea =;“sick TSI” Pasteurella multocida
-“musty” “mushroom” odor -get from animal contact esp. cat/dog bite (Cellulitis), respiratory tract/eye infect Pasteurella
-leading cause of endocarditic within genus -Cat/Oxi =, Nitrate +, glucose/lactose fermented Hemophilus aphrophilus
-dental infections; Cat + Oxi = -poor growth on SBA/CHOC, adhere to agar, “pointed star” Actinobacillus actinomycetemconitans
-associated exclusively with endocarditis -rosettes on gram stain -Oxi/Ind + ; cat/Nit = Cardiobacterium hominis
-fist fight/bite “clenched fist wound” -pits agar, “bleach odor” -Oxi/Nit + ; non motile ; non saccharolytic Eikenella corrodens
-plumb rods in chains; Oxi + Cat = ; ferment glucose -flora of pharynx in young children; Remel Rapid NH Kingella spp. denitrificans/kingae
-tularemia(get from animal carcasses, biting ticks),use safety BSC III Francisella tulrensis
-BCYE (cystine/iron), CHOC+, slow growth (25 days) -sm green drop like colonies -Cat + , ELISA, PCR Francisella tulrensis
-most normal flora of upper respiratory tract -DO NOT refrigerate,“blood lover”, Oxi/Cat + Haemophilus
-tiny, nonmotile gram neg. coccobacilli - X (hemin), V (NAD): CHOC provides both Haemophilus
-affects children spread by droplets and close contact -needs X & V H. influenza
-virulent factors:polysaccharide capsule, capsule type B , IgA proteases -non-encapsulated strain: adherence, lipoliosaccharide (LOS) H. influenza
satellites around S. aureus on SBA (sm translucent colonies) H. influenza
- “pink eye”(conjunctivitis) -Brazilian purpuric fever -needs X & Y -Remel Rapid NH ID (4 hrs) H. aegyptius
-STD: genital ulcers/chancres/chancroid -X factor only -plate immediately, high humidity, growth 2-14 days, “school of fish”, Cat = , Oxi + H. ducreyi
V only H. parainfluenzae
-X & V -Beta hemolysis in rabbit/horse BA H. haemolyticus
gram neg. diplococcic ”kidney shaped” Oxi/Cat +, CHOC for isolation Neisseria
sexually transmitted N. gonorrhaeae
-males asymptomatic - PID (pelvic inflammatory disease) N. gonorrhaeae
-virulence: pili, IgA protease, capsule -specimen: genital sites, use Dacon/Rayon swab, plate immediately onto CO2 generators & bags N. gonorrhaeae
-MTM (Modified Thayer Martin)-antibiotics inhibits other bacteria -ID: gram neg. diplococcic, CHOC growth, not SBA, Cat/Oxi + N. gonorrhaeae
-affects adolescents(college dorms) -vaccine -community acquired meningitis N. Meningitidis
-strains: A,B,C,Y, W135 -virulence: pili, capsule, endotoxins, IgA protease -specimen: CSF, blood, skin scrapings N. Meningitidis
-ID: growth CHOC/SBA/MTM, Cat/Oxi + CTA (cystine trypticase soy)-red + -BBL crystal NH ID -DNA prove N. Meningitidis
-normal in respiratory tract, pathogenic if found in sterile site N. lactamica, N. sicca, N. subflava
-gram neg. diplococci -growth in SBA, no MAC -normal respiratory flora -Oxi/Cat + -Nitrate reduction + -Asacchrolytic Moraxella catarrhalis
causes abortion/sterility in animals Campylobacter jejuni
causes whooping cough Bordetella perussis
Created by: istophane