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micro 3 cont.
mirobiology health
| Question | Answer |
|---|---|
| Non acid fast, non sporulating, and noncapsulated bacilli | L. pneumophilia |
| Non fermentative Oxidase and Catalase Positive | L. pneumophilia |
| Cooling towers, air conditioning units, hottubs/spas, plumbing fixtures | L. pneumophilia |
| L. pneumophilia | The ability to survive and multiply in freeliving protozoa |
| Two Diseases,Legionnaires’Disease-Pontiac fever comes from | L. pneumophilia |
| Legionairres’ Disease | mortality rate 30% if not treated Symptoms depend on person- asymptomatic/life threat |
| More like influenza, no pneumonia, 0%mortality | Pontiac Fever |
| Isolation using special media Urine detect antigen detection | Pontiac Fever |
| Pontiac Fever | Survive and multiply in Macrophages(especially bronchoalvealor macrophages |
| Cysteine and iron/35C and CO2 Buffered Charcoal Yeast Agar | Pontiac Fever |
| Treatment is Erythromycin Clinical response within 48 hrs | Pontiac Fever |
| Zoonotic disease with worldwide distribution 6 species | Brucella |
| Most isolates grow of Brucella grow on bloodand chocolate agar,small,nonmotile.aerobes | Brucella |
| Catalase and Oxidase positive,Non sacchrolyticsurvive intracellularly in the RES system | Brucella |
| Normal flora in urinary and gastrointestinaltracts of goats, pigs, cows, and dogs | General char. of Brucella |
| brucella | Ingestion of contaminated dairy |
| Serology for Brucella | “Gold Standard” Febrile agglutinins |
| GramNegative,pleomorphic,coccobacilli,nonmotile,Catalase Positive | Pasteurella |
| Most are Oxidase Positive and FermentGlucose,weak acid on TSI slant | Pasteurella |
| the mucous membranes of the upper respiratory and gastrointestinal of mammals/birds | Pasteurella |
| Most common species isolated from humans | Pasteurella multocida |
| Pasteurella multocida | Grows on SBA and CHOC– Not on MAC |
| Oxidase and Catalase- Positive Indole Positive | Pasteurella multocida |
| Oral cavity of cats and dogs,Causative agents of several,Cattle, sheep, goats, poultry etc | Pasteurella multocida |
| Pasteurella multocida | Grey/Green, convex, nonhemolytic– “Mushroom” Odor |
| Urogenital flora,can cause Endocarditis, Bacteremia, Mixed flora wound infections | Hacek Organism |
| Hacek Organism | Most are slow growing 48-72 hrs, requires CO2 to grow |
| Leading cause of endocarditis within the Haemophilus sp. | Hemophilus aphrophilus |
| Catalase and Oxidase negative,Acid produced from glucose/lactose fermentation | Hemophilus aphrophilus |
| Endocarditis, Bacteremia, Wound infections, and Dental infections | Actinobacillus actinomycetemcomitans |
| Actinobacillus actinomycetemcomitans | Grows poorly on Blood and Chocolate- Catalase Positive and Oxidase negative |
| Normal flora of nose, mouth, and throat, may be present in the gastrointestinal tract | Cardiobacterium hominis |
| Pleomorphic, fastidious, gram negative bacillus Associated with endocarditis | Cardiobacterium hominis |
| Oxidase and Indole Positive Catalase and Nitrate Negative | Cardiobacterium hominis |
| Normal flora of oral and bowel cavities in the body | Eikenella corrodens |
| Fastidious gram negative coccobacilli has a strong bleach-like odor | Eikenella corrodens |
| Oxidase positive and Nitrate positive Nonmotile | Eikenella corrodens |
| Normal flora of human upper respiratory tract and genitourinary tract | Kingella spp |
| Kingella spp | Plump Gram Negative Rods |
| Kingella spp | Oxidase Positive and Catalase Negative ±Ferment Glucose |
| Transmission: handling animals or animalcarcasses,Highly contagious and invasive | Francisella tularensis |
| Cystine and Iron,Buffered Charcoal Yeast Extract Agar,choc.agar | Francisella tularensis |
| Francisella tularensis | Faint staining, Gram negative coccobacilliSmall, greenish, drop like colonies |
| Haemophilus sp | Most members are non pathogenic oropportunistic pathogens |
| normal flora of the upper respiratory tractSusceptible to temperature change | Haemophilus sp |
| Tiny Gram Negative Coccobacilli,Non-MotileFacultative Anaerobes | Haemophilus sp |
| derived from Greek for “Bloodlover” | Haemophilus |
| X factor- what is it made of– V Factor- what is it made of | X---hemin, hematinV---nicotinamide-adenine dinucleotide |
| Major clinical pathogen,Normal flora of the upper respiratory tract | Haemophilus influenzae |
| Vaccine- single biggest impact on pediatricsin the last 20 years | Haemophilus influenzae |
| Polysaccharide capsule (7 serogroupsexist)What type most common | Capsule Type B is the most clinicallysignificant/virulent |
| Non-encapsulated strains | Adherance: ability to adhere to mucosalsurfaces |
| Non-encapsulated StrainsClinical Disease | Causes localized infections |
| Requires 3-5% CO2Satellites around S. aureus on SBA | Non-encapsulated Strains |
| Haemophilus aegyptius | Conjucivitis “Pink Eye”Brazilian Purpuric Fever |
| Requires X/V GrowsinChoc,chemical Differentiation require | Haemophilus aegyptius |
| 4 hour identification of over 30 taxaincluding Neisseria, Moraxella,Haemophilus, | Remel Rapid NH ID |
| Not normal flora STDGenital tract pathogen, | Haemophilus ducreyi |
| 4-14 days after incubation from the time ofinfection,Painful lesion, no edema | Haemophilus ducreyi |
| Localized endemic outbreaks occur in isolatedSTD and prostitution populations | Chancroid is rare in the United States |
| Does not need V factor– Fastidious will not satellite on BAP | Chancroid |
| Gram negative coccobacillus- “Schoolof Fish” pattern | Chancroid |
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