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AMS Testing

Microbiology ppt 2 - Antimicrobial Susceptibility Testing

QuestionAnswer
Standardized Components of AMS -Bacterial inoculum size -Growth medium -Incubation atmosphere/temperature/duration -Antimicrobial concentration
Limitations of Standardization Cannot predict patient outcome due to: -Antibiotic diffusion into tissues -Drug interactions and interference -Status ot patient immune systems
Organism suspension must have turbidity equivalent to... 0.5 McFarland standard
MIC Minimal Inhibitory Concentration = the lowest antimicrobial concentration that will completely inhibit visible bacterial growth.
Incubation time, atmosphere and temperature for routine, non- fastidious bacteria (K-B technique) 16-18 hours at 35 degrees Celsius, room air
What should you check before reading your K-B results Purity plate
When can a haze of growth within The Inhibition Zone be Ignored? 1) Swarming of Proteus spp. Measure from where colonies are obviously inhibited. 2) When testing sulfonamides and trimethoprim
Fastidious organisms that would require modifications of KB Haemophilus influenzae, Neisseria menigitidis, neisseria gonorrhoeae & Streptococcus pneumoniae. All require CO2 enviroments and all need different media.
What KB modifications would Streptococcus pneumoniae require? MH Plate with blood
Advantages of Etest Provides MIC data when the level of resistance is clinically important. Ex - Penicillin and cephalosporins against S. pneumoniae.
Created by: moreandmore
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