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Lab Quiz #1

1-1, 1-3, 1-4, 3-1, 3-3

culture a medium that contains living microbes
pure culture a culture that contains a single species
aseptic free from contamination caused by harmful bacteria, viruses, or other microorganisms; surgically sterile or sterilized.
broths used to grow microbes when fresh cultures or large numbers of cells are required; are also used in microbial identification
agar slants used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks
plated media typically used for obtaining isolation of species, differential testing, and quantifying bacterial densities (all require aseptic inoculation)
resolution clarity of an image (through a light microscope); ______ improves as the resolving power is made smaller
limit of resolution (aka resolving power) is an actual measurement of how far apart two points must be for the microscope to view them as being separate; the best achieved by a light microscope is 0.2 um
condenser lens concentrates the light and makes illumination of the specimen more uniform
total magnification (formula question) magnification by the objective lens x magnification by the ocular lens
numerical aperture is the measure of a lens's ability to "capture" light coming from the specimen and use it to make the image; is marked on the lens (0.10, 0.25, 0.65, 1.25)
dark-field microscopy a special condenser is used so only the light reflected off the specimen enters the objective; the appearance is of a brightly lit specimen against a dark background (often w/ better resolution then bright-field microscopy)
phase contrast microscopy uses optical components to exploit differences in the refractive indices of water & cytoplasmic components to produce contrast; contrast is provided by differences in light intensity; specimen appears various levels of "dark" against bright background
fluorescence microscopy uses a fluorescent dye that emits fluoresces when illuminated w/ ultra-violet radiation
Objective lense scanning = x4, low power = x10, high dry = 40x, oil immersion = 100x
Created by: microstudent
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