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Lab Quiz #1
1-1, 1-3, 1-4, 3-1, 3-3
| Term | Definition |
|---|---|
| culture | a medium that contains living microbes |
| pure culture | a culture that contains a single species |
| aseptic | free from contamination caused by harmful bacteria, viruses, or other microorganisms; surgically sterile or sterilized. |
| broths | used to grow microbes when fresh cultures or large numbers of cells are required; are also used in microbial identification |
| agar slants | used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks |
| plated media | typically used for obtaining isolation of species, differential testing, and quantifying bacterial densities (all require aseptic inoculation) |
| resolution | clarity of an image (through a light microscope); ______ improves as the resolving power is made smaller |
| limit of resolution (aka resolving power) | is an actual measurement of how far apart two points must be for the microscope to view them as being separate; the best achieved by a light microscope is 0.2 um |
| condenser lens | concentrates the light and makes illumination of the specimen more uniform |
| total magnification (formula question) | magnification by the objective lens x magnification by the ocular lens |
| numerical aperture | is the measure of a lens's ability to "capture" light coming from the specimen and use it to make the image; is marked on the lens (0.10, 0.25, 0.65, 1.25) |
| dark-field microscopy | a special condenser is used so only the light reflected off the specimen enters the objective; the appearance is of a brightly lit specimen against a dark background (often w/ better resolution then bright-field microscopy) |
| phase contrast microscopy | uses optical components to exploit differences in the refractive indices of water & cytoplasmic components to produce contrast; contrast is provided by differences in light intensity; specimen appears various levels of "dark" against bright background |
| fluorescence microscopy | uses a fluorescent dye that emits fluoresces when illuminated w/ ultra-violet radiation |
| Objective lense | scanning = x4, low power = x10, high dry = 40x, oil immersion = 100x |
Created by:
microstudent
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