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Non ferm.bacilli
micro test 3
| Question | Answer |
|---|---|
| Non fermenters | Reqiure oxygen,cannot ferment glucose and form weak acids |
| Cannot use carbohydrates in the absence of oxygen | Nonsaccharolytic |
| Use compounds other than carbohydrates for energy | Nonoxidizers |
| Nonsaccharolytic | Nonreactive on TSI slants |
| detects small amount of acid,1% carbohydrate,0.2%peptones | OF medium |
| Suspect of a Nonfermentor | Growth better on blood plate than MAC,oxidase pos,TSI K/K |
| Detemines wheather an organism uses carbohydrate to produce acid product | OF test |
| Infections occur where moisture accumulates,eyes,ears catheters | P.aeruginosa |
| P.Aeroginosa | Chronic lung infection, CF, |
| UTI's, pulmonary disease, endocarditis and septicemia | P.aeruginosa |
| P.aeruginosa | grape like odor |
| P. Aeruginosa | Large colonies,grape like odor,oxidase positive,pyocyanin |
| Pseudomnas means what in greek | False unit |
| Produces pyoverdin, does not grow at 42C, | P.Fluorescens and P.Putida |
| P.Stutzeri | Soil denitrifier,cause of otitis media and septic arthritis |
| How many species are in Burkholderia | 50 species |
| B.Cepacia is found where | IV fluids,detergents and disinfectants |
| Cause of onion bulb rot | B.cepacia |
| Opportunistic and nosocmiaol pathogen and isolates itself from alcohol/iodine | B.CEPACIA |
| Growth on Blood, chocolate and MAC | B.cepacia |
| Yellowish pigment and dirt like odor | B.cepacia |
| Primary in horses,goats,sheep and donkeys | B.Mallei |
| Only non-motile organism in Burkholderia and Pseudomonas species | B.Mallei |
| B.Psuedomallei | Aggressive granulomatous pulminary disease |
| 3rd most commonly isolated non-fermenter infections | Stenotrophomonas Maltophilia |
| Aerobic,motile by polar flagella,resistance to antimicrobial agents | S. Maltophilia |
| S.Maltophilia | Strongly oxidizes maltose, amonia odor |
| Aerobic nonfermentative gram neg.bacilli,grows on MAC,oxidase neg. nonmotile | Acineobacter |
| Acineobacter | Produces purple pigment on MAC |
| Non-pathogenic to healthy people,most infections in immunocompromised | Acineobacter |
| A.baumannii | Saccharolytic |
| A.Iwoffii | Nonsaccharolytic |
| Pathogen,Isolation indicates contamination/colonization rather infection | A.Iwoffii |
| Found in plankton,brackish, and salt water water, borne illness | Vibrio Cholerae |
| Acute diarrheal disease that is spread mainly through contaminated water | Cholera |
| Attached to intestinal villi by pilli | Cholera |
| Cholera Toxin or Choleragen | Massive outpouring of Rice water stools |
| Vibro sp. | Natural inhabitants of seawater,Most require increased sodium chloride for growth |
| Oxidase Positive, Facultative Anaerobes, Non-spore forming Gram Negative Bacilli | Vibro sp. |
| Vibrio cholerae Epidemiology | Water borne illness Found in plankton, fresh, brackish,and salt water |
| is an acute diarrheal disease that is spread mainly through contaminated water | Cholera |
| Colonizes the gastrointestinal tract | Cholera |
| Vibrio cholera 01-Two Biotypes | 1.Classic--Nonhemolytic, VP Negative2.El Tor---Beta hemolytic, VP Positive |
| NLF on MAC,Yellow Colonies on TCBS Vibrio cholera | Vibrio cholera |
| Vibrio parahaemolyticus | Gastroenteritis from seafood (Shell Fish),Green on TCBS, Non sucrose fermenter |
| Require 1-2% NaCl for growth,Cause gastroenteritis, wound infections,septicemia | Halophilic Vibrios |
| Vibrio vulnificus | Septicemia from raw shellfish,Lactose fermenter |
| Aeromonas sp. | Oxidase positive, glucose fermenting, gram negative rods |
| Cause diseases in warm bloodedand cold blooded animals | Aeromonas sp. |
| Straight, not curved rods,single polar,Grow in range of temp from 0-42C | Aeromonas sp. |
| Aeromonas hydrophilia | Water loving,Grows on MAC, Indole Positive, Waterborne Illness |
| Produces aerolysin,Cytotoxic enterotoxin,Causes tissue damage in fish | Aeromonas hydrophilia |
| MAC and SBA,Usually beta-hemolytic,Positive indole and oxidase | Aeromonas hydrophilia |
| Aeromonas hydrophilia | resistant to Penicillin,ampicillin, carbenicillin,cephalothin |
| Plesiomonas sp. | Oxidase positive, glucose fermenting, facultatively anaerobic gram negative rods |
| Potential cause of enteric disease in humans, carried on cold blooded animals | Plesiomonas sp |
| Causes gastritis | Plesiomonas shigelloides |
| not recovered on routine media Both are small, curved, motile,gram negative bacilli | Campylobacter and Helicobacter |
| S Shaped,Microaerophilic (Reduced Oxygen)Oxidase Positive and Nonfermentative | Campylobacter spp. |
| are pathogenic and are associated with a wide variety of human diseases | Campylobacter spp |
| Campylobacter spp | cause abortion in domestic animals such as cattle, sheep, swine |
| One of the most common causes of bacterial gastroenteritis in numerous parts of US | C. jejuni |
| Bloody stool with high number of WBC’s,Lasts for 7-10 days,Can be fatal | C. jejuni |
| Ingestion of contaminated food or water | C. jejuni |
| Special atmospheric and temperature needs:42C,5% O2, 10% CO2, 85% N2 | C. jejuni |
| C. jejuni | Curved gram negative bacilli Sea gull shape |
| Helicobacter spp | Curved, microaerophilic gram negative rods,Most species- strong urease |
| Natural habitat is human stomach | H. pylori |
| Coverts urea to ammonia,bicarbonate,Elicits a powerful immuneresponse- Ulcer | H. pylori |
| Positive- Oxidase, Catalase,Urease | H. pylori |
| Alcaligenes | Tiny motile gram negative rods Oxidase positive and grows on MAC |
| Reduces nitrates to nitrites Found in water and soil | Alcaligenes |
| Most commonly isolated species | Alcaligenes faecalis |
| Alcaligenes faecalis | Opportunistic infections of blood, CSF,UTI, and wound infection |
| irregular edges (feather edge) on BAP “Fruity” plate odor,Grows in 6.5% NaCl | Alcaligenes faecalis |
| Small Coccobacilli,Fastidous, Obligate aerobes | Bordetella species |
| B. pertussis and B. parapertussis are | non motile, |
| Worldwide 60 million cases with,500,000 deaths,US occurs August to November | B. pertussis |
| Exotoxin enters targetcells and activates cAMP | Pertussis toxin- |
| causes ciliatedepithelial cells destruction | Tracheal cytotoxin |
| a cell surface,protein binds to host cell surface | Hemoaggutination |
| Whooping Cough | B. pertussis |
| Potato infusion agarand sheep blood with methicillin | Bordet-Genou |
| Charcoal agar with 10%Horse Blood and caphalexin | Regan-Lowe |
| B. parapertussis | Causes pertussis with milder symptoms– Nontoxigenic strain |
| Kennel cough in dogs, human infectionsresults from transmission from animal | B. bronchisepica |
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hintonworld
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