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Non ferm.bacilli

micro test 3

Non fermenters Reqiure oxygen,cannot ferment glucose and form weak acids
Cannot use carbohydrates in the absence of oxygen Nonsaccharolytic
Use compounds other than carbohydrates for energy Nonoxidizers
Nonsaccharolytic Nonreactive on TSI slants
detects small amount of acid,1% carbohydrate,0.2%peptones OF medium
Suspect of a Nonfermentor Growth better on blood plate than MAC,oxidase pos,TSI K/K
Detemines wheather an organism uses carbohydrate to produce acid product OF test
Infections occur where moisture accumulates,eyes,ears catheters P.aeruginosa
P.Aeroginosa Chronic lung infection, CF,
UTI's, pulmonary disease, endocarditis and septicemia P.aeruginosa
P.aeruginosa grape like odor
P. Aeruginosa Large colonies,grape like odor,oxidase positive,pyocyanin
Pseudomnas means what in greek False unit
Produces pyoverdin, does not grow at 42C, P.Fluorescens and P.Putida
P.Stutzeri Soil denitrifier,cause of otitis media and septic arthritis
How many species are in Burkholderia 50 species
B.Cepacia is found where IV fluids,detergents and disinfectants
Cause of onion bulb rot B.cepacia
Opportunistic and nosocmiaol pathogen and isolates itself from alcohol/iodine B.CEPACIA
Growth on Blood, chocolate and MAC B.cepacia
Yellowish pigment and dirt like odor B.cepacia
Primary in horses,goats,sheep and donkeys B.Mallei
Only non-motile organism in Burkholderia and Pseudomonas species B.Mallei
B.Psuedomallei Aggressive granulomatous pulminary disease
3rd most commonly isolated non-fermenter infections Stenotrophomonas Maltophilia
Aerobic,motile by polar flagella,resistance to antimicrobial agents S. Maltophilia
S.Maltophilia Strongly oxidizes maltose, amonia odor
Aerobic nonfermentative gram neg.bacilli,grows on MAC,oxidase neg. nonmotile Acineobacter
Acineobacter Produces purple pigment on MAC
Non-pathogenic to healthy people,most infections in immunocompromised Acineobacter
A.baumannii Saccharolytic
A.Iwoffii Nonsaccharolytic
Pathogen,Isolation indicates contamination/colonization rather infection A.Iwoffii
Found in plankton,brackish, and salt water water, borne illness Vibrio Cholerae
Acute diarrheal disease that is spread mainly through contaminated water Cholera
Attached to intestinal villi by pilli Cholera
Cholera Toxin or Choleragen Massive outpouring of Rice water stools
Vibro sp. Natural inhabitants of seawater,Most require increased sodium chloride for growth
Oxidase Positive, Facultative Anaerobes, Non-spore forming Gram Negative Bacilli Vibro sp.
Vibrio cholerae Epidemiology Water borne illness Found in plankton, fresh, brackish,and salt water
is an acute diarrheal disease that is spread mainly through contaminated water Cholera
Colonizes the gastrointestinal tract Cholera
Vibrio cholera 01-Two Biotypes 1.Classic--Nonhemolytic, VP Negative2.El Tor---Beta hemolytic, VP Positive
NLF on MAC,Yellow Colonies on TCBS Vibrio cholera Vibrio cholera
Vibrio parahaemolyticus Gastroenteritis from seafood (Shell Fish),Green on TCBS, Non sucrose fermenter
Require 1-2% NaCl for growth,Cause gastroenteritis, wound infections,septicemia Halophilic Vibrios
Vibrio vulnificus Septicemia from raw shellfish,Lactose fermenter
Aeromonas sp. Oxidase positive, glucose fermenting, gram negative rods
Cause diseases in warm bloodedand cold blooded animals Aeromonas sp.
Straight, not curved rods,single polar,Grow in range of temp from 0-42C Aeromonas sp.
Aeromonas hydrophilia Water loving,Grows on MAC, Indole Positive, Waterborne Illness
Produces aerolysin,Cytotoxic enterotoxin,Causes tissue damage in fish Aeromonas hydrophilia
MAC and SBA,Usually beta-hemolytic,Positive indole and oxidase Aeromonas hydrophilia
Aeromonas hydrophilia resistant to Penicillin,ampicillin, carbenicillin,cephalothin
Plesiomonas sp. Oxidase positive, glucose fermenting, facultatively anaerobic gram negative rods
Potential cause of enteric disease in humans, carried on cold blooded animals Plesiomonas sp
Causes gastritis Plesiomonas shigelloides
not recovered on routine media Both are small, curved, motile,gram negative bacilli Campylobacter and Helicobacter
S Shaped,Microaerophilic (Reduced Oxygen)Oxidase Positive and Nonfermentative Campylobacter spp.
are pathogenic and are associated with a wide variety of human diseases Campylobacter spp
Campylobacter spp cause abortion in domestic animals such as cattle, sheep, swine
One of the most common causes of bacterial gastroenteritis in numerous parts of US C. jejuni
Bloody stool with high number of WBC’s,Lasts for 7-10 days,Can be fatal C. jejuni
Ingestion of contaminated food or water C. jejuni
Special atmospheric and temperature needs:42C,5% O2, 10% CO2, 85% N2 C. jejuni
C. jejuni Curved gram negative bacilli Sea gull shape
Helicobacter spp Curved, microaerophilic gram negative rods,Most species- strong urease
Natural habitat is human stomach H. pylori
Coverts urea to ammonia,bicarbonate,Elicits a powerful immuneresponse- Ulcer H. pylori
Positive- Oxidase, Catalase,Urease H. pylori
Alcaligenes Tiny motile gram negative rods Oxidase positive and grows on MAC
Reduces nitrates to nitrites Found in water and soil Alcaligenes
Most commonly isolated species Alcaligenes faecalis
Alcaligenes faecalis Opportunistic infections of blood, CSF,UTI, and wound infection
irregular edges (feather edge) on BAP “Fruity” plate odor,Grows in 6.5% NaCl Alcaligenes faecalis
Small Coccobacilli,Fastidous, Obligate aerobes Bordetella species
B. pertussis and B. parapertussis are non motile,
Worldwide 60 million cases with,500,000 deaths,US occurs August to November B. pertussis
Exotoxin enters targetcells and activates cAMP Pertussis toxin-
causes ciliatedepithelial cells destruction Tracheal cytotoxin
a cell surface,protein binds to host cell surface Hemoaggutination
Whooping Cough B. pertussis
Potato infusion agarand sheep blood with methicillin Bordet-Genou
Charcoal agar with 10%Horse Blood and caphalexin Regan-Lowe
B. parapertussis Causes pertussis with milder symptoms– Nontoxigenic strain
Kennel cough in dogs, human infectionsresults from transmission from animal B. bronchisepica
Created by: hintonworld