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Biology

Biomolecules

Term/definition Question/answer
Macromolecules made up of large molecular weight (insoluble in chloro-acetic acid)
Micromolecules less molecular weight (soluble in chloro-acetic acid)
Enzymes (2 types) increases the rate of reaction w/o undergoing any change in itself, they are proteinaceous in nature
Simple enzymes made up of only proteins; e.g- pepsin, trypsin
Conjugated enzymes made up both protein and non-protein part, attached with proteins
What is the protein part in conjugated enzymes known as? apoenzymes
What is non-protein part in conjugated enzymes known as? co-factors (2 types)
Organic CF 1. co enzymes: easily get separated 2. prosthetic group: tightly packed, can't be separated easily
Inorganic CF they initiates the reaction, e.g- metallic ions
Activation energy minimum energy taken up by substrate to work
Substrate gives rise to products
Working of enzymes (induced-fit theory) enzyme + substrate → enzyme substrate complex (remain together temporarily) - (substrate changes into product) → Enzyme + Product
Coordination bond bond between S1 and S2
Active site where substrates get attached
Classification of enzymes 1. intracellular enzymes (endogenous enz) 2. extracellular enzymes (exogenous enz)
Endogenous enzymes present inside the cell
Exogenous enzymes present outside the cell
Induced-fit theory enzymes undergo confirmational change so that S1 and S2 can come closer to each other
Turn over no. of molecules of substrate which can be used by enzymes
Factors responsible for enzyme activities 1. temperature 2. pH 3. concentration of substrate
How does temperature affects the enzyme activities? high temp: enzymes→ denatured low temp: enzymes → inactive
Optimal temperature for enzyme activities 25 deg C to 40 deg C
How does pH affects the enzyme activities? as the pH value is increased above or decreased below the optimum pH the enzyme activity decreases
How does concentration affects the enzyme activities? more substrate → enzyme activity increases
Factors inhibiting the the working of enzymes 1. high & low temperature 2. competitive inhibition 3. non-competitive inhibition
Competition inhibitator if it is present with substrate, the morphology is same as the morphology of substrate (E + S + Ci) hence will suppress the activities of enzymes - attached to the main site
Non-competition inhibitator not attached to the main site and will decrease the turn over no. IN BOTH THE CASES , ENZYMES DOES NOT WORK PROPERLY
Created by: Aliya_02
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