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TOPIC 3

MITOCHONDRIA

QuestionAnswer
how does centrifugation work explain
what is the role of the plasma membrane • cell surrounded by plasma membrane (lipid bilayer) • one or more lipid bilayers surround the organelles
explain some origins of organelles diagrams
what is the mitochondria and its function • up to 1μm wide and 10μm long • most cells have hundreds • double membrane leads to multiple compartments • in both plants and animals
list some fun facts of the mitochondria • up to 1μm wide and 10μm long • most cells have hundreds • double membrane leads to multiple compartments • in both plants and animals
how does the mitochondria produce energy for the cell explain
how does catabolism in mitochondria work acetyl CoA
what are the compartments in the mitochondria & their roles inner membrane outer membrane matrix
what is glycolysis • process where glucose is oxidized to pyruvate glucose + 2 NAD+ + 2 ADP + 2 Pi  2 pyruvate + 2 NADH + 2 H+ + 2 ATP
what is the citric acid cycle • important for energy production • what are the major products? • where is the pyruvate from? Fig. 10-7
what are the products of TCA cycle acetyl CoA + 3 NAD+ + FAD + ADP + Pi  2 CO2 + 3 NADH + FADH2 + CoA-SH + ATP FROM 1 GLUCOSE MOLECULE: glucose + 10 NAD+ + 2 FAD + 4 ADP + 4 Pi  6 CO2 + 10 NADH + 2 FADH2 + 4 ATP
explain the e- transport chain and why we utilize it diagram
stepwise transfer of e-s (4 complexes) • this is the mitochondrial ETC • four complexes
how many protons are pumped diagram
what is the chemiosmotic hypothesis by Peter Mitchell • Transfer of electrons in the ETC creates a proton gradient. • Proton gradient leads to chemical gradient ([H+] or pH) and charge gradient proton motive force. Proton motive force (Dp) = chemical gradient (DpH) + charge gradient (D • Redistribution
what ist= the definitive experiment Light causes protons to be pumped into the synthetic vesicle by this protein Bovine heart ATP synthase Synthetic membrane vesicle Biochemistry, 7th edition Figure 18.23 the definitive experiment
explain proton motion drives rotation diagram
proton path Each proton must travel a full circle before being released into the matrix. Biochemistry, 7th edition Figure 18.32
explain ATP synthase (4 steps)
what is ATP yield 360o rotation of the g-subunit yields 3 ATP. • If the c ring has 10 subunits then each ATP needs 10/3 = 3.33 or ~3 protons to be transported. • Actual value closer to 4 protons/ATP
explain oxidative phosphorylation this process is called chemiosmotic coupling 2 stage process: 1. high energy e-s are used to pump H+ across a membrane 2. H+ flows down the gradient through ATP synthase Fig. 10-26 From the 9th Edition
what is the uncoupling of oxidative phosphorylation • poisons, such as cyanide, uncouple oxidative phosphorylation, resulting in a loss of ATP production • cyanide acts by binding cytochrome oxidase • dinitrophenol can shuttle protons across membranes (shown at left) Fig. 10B-1
what is the overall energy production 1 GLUCOSE MOLECULE glucose + 2 NAD+ + 2 ADP + 2 Pi  2 pyruvate + 2 NADH + 2 H+ + 2 ATP glucose + 10 NAD+ + 2 FAD + 4 ADP + 4 Pi  6 CO2 + 10 NADH + 2 FADH2 + 4 ATP 10NADH + 10H+ + 2FADH2 + 6O2 + ~30ADP + + ~30 Pi  10NAD+ + 2 FAD + 12H2O + ~30ATP
what is the complete oxidation of glucose • ATP is the cellular currency of energy • 30 ATP molecules generated per glucose Table 21.1
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