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TOPIC 3
MITOCHONDRIA
| Question | Answer |
|---|---|
| how does centrifugation work | explain |
| what is the role of the plasma membrane | • cell surrounded by plasma membrane (lipid bilayer) • one or more lipid bilayers surround the organelles |
| explain some origins of organelles | diagrams |
| what is the mitochondria and its function | • up to 1μm wide and 10μm long • most cells have hundreds • double membrane leads to multiple compartments • in both plants and animals |
| list some fun facts of the mitochondria | • up to 1μm wide and 10μm long • most cells have hundreds • double membrane leads to multiple compartments • in both plants and animals |
| how does the mitochondria produce energy for the cell | explain |
| how does catabolism in mitochondria work | acetyl CoA |
| what are the compartments in the mitochondria & their roles | inner membrane outer membrane matrix |
| what is glycolysis | • process where glucose is oxidized to pyruvate glucose + 2 NAD+ + 2 ADP + 2 Pi 2 pyruvate + 2 NADH + 2 H+ + 2 ATP |
| what is the citric acid cycle | • important for energy production • what are the major products? • where is the pyruvate from? Fig. 10-7 |
| what are the products of TCA cycle | acetyl CoA + 3 NAD+ + FAD + ADP + Pi 2 CO2 + 3 NADH + FADH2 + CoA-SH + ATP FROM 1 GLUCOSE MOLECULE: glucose + 10 NAD+ + 2 FAD + 4 ADP + 4 Pi 6 CO2 + 10 NADH + 2 FADH2 + 4 ATP |
| explain the e- transport chain and why we utilize it | diagram |
| stepwise transfer of e-s (4 complexes) | • this is the mitochondrial ETC • four complexes |
| how many protons are pumped | diagram |
| what is the chemiosmotic hypothesis by Peter Mitchell | • Transfer of electrons in the ETC creates a proton gradient. • Proton gradient leads to chemical gradient ([H+] or pH) and charge gradient proton motive force. Proton motive force (Dp) = chemical gradient (DpH) + charge gradient (D • Redistribution |
| what ist= the definitive experiment | Light causes protons to be pumped into the synthetic vesicle by this protein Bovine heart ATP synthase Synthetic membrane vesicle Biochemistry, 7th edition Figure 18.23 the definitive experiment |
| explain proton motion drives rotation | diagram |
| proton path | Each proton must travel a full circle before being released into the matrix. Biochemistry, 7th edition Figure 18.32 |
| explain ATP synthase | (4 steps) |
| what is ATP yield | 360o rotation of the g-subunit yields 3 ATP. • If the c ring has 10 subunits then each ATP needs 10/3 = 3.33 or ~3 protons to be transported. • Actual value closer to 4 protons/ATP |
| explain oxidative phosphorylation | this process is called chemiosmotic coupling 2 stage process: 1. high energy e-s are used to pump H+ across a membrane 2. H+ flows down the gradient through ATP synthase Fig. 10-26 From the 9th Edition |
| what is the uncoupling of oxidative phosphorylation | • poisons, such as cyanide, uncouple oxidative phosphorylation, resulting in a loss of ATP production • cyanide acts by binding cytochrome oxidase • dinitrophenol can shuttle protons across membranes (shown at left) Fig. 10B-1 |
| what is the overall energy production | 1 GLUCOSE MOLECULE glucose + 2 NAD+ + 2 ADP + 2 Pi 2 pyruvate + 2 NADH + 2 H+ + 2 ATP glucose + 10 NAD+ + 2 FAD + 4 ADP + 4 Pi 6 CO2 + 10 NADH + 2 FADH2 + 4 ATP 10NADH + 10H+ + 2FADH2 + 6O2 + ~30ADP + + ~30 Pi 10NAD+ + 2 FAD + 12H2O + ~30ATP |
| what is the complete oxidation of glucose | • ATP is the cellular currency of energy • 30 ATP molecules generated per glucose Table 21.1 |