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ap bio unit 4 part 3
bacteria
| Question | Answer |
|---|---|
| typically reproduce by asexual _____ _______ | binary fission |
| binary fission | circular DNA chromosome attaches to cell membrane and replicates, then bacteria cleaves in half with one chromosome in each half |
| haploid | contains one set of DNA instructions |
| diploid | 2 sets of DNA instructions |
| plasmids | small, circular accessory molecules of DNA |
| conjugation (F+/F-) | a form of genetic material exchange in procaryotes (bacteria sex) |
| F+ | contains sex factor plasmid |
| F- | does not contain sex factor plasmid |
| sex factor plasmid | - contains info for pilus formation and info to alter cell wall - contains genes that have nothing to do with sex (help break down food, resistance to drugs, etc) |
| tranformation | when bacteria take in pieces of genetic info from surrounding environment (like what happened in Griffith's experiment) |
| transduction | - somewhat opposite process of viral DNA moving into the host DNA - the virus assembles host DNA and carries it to another host |
| transposons | "jumping genes" - exist both in eu/prokaryotes sequences of DNA that r able to copy themselves and move locations - sequences are like introns that won't go away "introns from hell" - causes genetic variability |
| defense system | the reason why phages haven't exterminated all the bacteria |
| restriction endonuclease enzymes | found in bacteria that cut up foreign DNA at specific sites in DNA (restriction enzymes) (deactivates viral DNA) |
| conjugation between an F+ E.coli and an F- cell results in the F- cell becoming F+ | true |
| the sex factor in E. coli is not autonomous because... | it can be integrated into bacterial chromosomes |
| in bacterial conjugation, usually only part of a chromosome is transferred from the donor cell to the recipient cell | true |
| studies of Hfr conjugation suggested that some genes can move | true |
| during transduction... | a virus transfers bacterial genes from one bacterium to another |
| restriction endonucleases | are enzymes used to cut DNA at specific sites, creating sticky ends |
| gene cloning, using radioactive cDNA can be used to map genes | true |
| a plasmid is... | self-replicating nonchromosomal circle of DNA |
| bacteria that harbor a provirus are said to be... | lysogenic |
| the process by which a virus transfers genes from one bacterial cell to another is called... | transduction |
| one problem with using the artificial transformation technique to insert genes from one organism into cells of another kind of organism such as bacteria is that... | the foreign genes generally contain introns that bacteria are unable to remove |
| gene cloning steps 1-3: | 1. mRNA molecules coding for the desired product are isolated 2. a cDNA copy of mRNA is made using reverse transcriptase and the DNA strand is replicated 3. restriction endonucleases are used to cut DNA and plasmid, producing sticky ends |
| gene cloning steps 4-5: | 4. the plasmid DNA and cloned gene are joined and sealed by ligase 5. the plasmid is inserted into the bacterial host cell |